DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions

Jerry M. Orosz; James G. Wetmur

doi:10.1002/bip.1977.360160603

DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions

Jerry M. Orosz
, James G. Wetmur

Icahn School of Medicine at Mount Sinai

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

DNA melting temperatures and renaturation rates have been determined for Me₂Et₂NBr and a series of RMe₃NBr and REt₃NBr solvents where R is a linear hydrocarbon chain. The point of independence of DNA melting temperature on base composition has been investigated for each solvent system. Renaturation rates are compared with those found in other concentrated salt solutions. Solvent mixtures which accelerate DNA renaturation have also been investigated.

Original language	English
Pages (from-to)	1183-1199
Number of pages	17
Journal	Biopolymers
Volume	16
Issue number	6
DOIs	https://doi.org/10.1002/bip.1977.360160603
State	Published - Jun 1977

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@article{9a4ccfd9e214470b9a34649534141223,

title = "DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions",

abstract = "DNA melting temperatures and renaturation rates have been determined for Me2Et2NBr and a series of RMe3NBr and REt3NBr solvents where R is a linear hydrocarbon chain. The point of independence of DNA melting temperature on base composition has been investigated for each solvent system. Renaturation rates are compared with those found in other concentrated salt solutions. Solvent mixtures which accelerate DNA renaturation have also been investigated.",

author = "Orosz, \{Jerry M.\} and Wetmur, \{James G.\}",

year = "1977",

month = jun,

doi = "10.1002/bip.1977.360160603",

language = "English",

volume = "16",

pages = "1183--1199",

journal = "Biopolymers",

issn = "0006-3525",

publisher = "John Wiley and Sons Inc.",

number = "6",

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TY - JOUR

T1 - DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions

AU - Orosz, Jerry M.

AU - Wetmur, James G.

PY - 1977/6

Y1 - 1977/6

N2 - DNA melting temperatures and renaturation rates have been determined for Me2Et2NBr and a series of RMe3NBr and REt3NBr solvents where R is a linear hydrocarbon chain. The point of independence of DNA melting temperature on base composition has been investigated for each solvent system. Renaturation rates are compared with those found in other concentrated salt solutions. Solvent mixtures which accelerate DNA renaturation have also been investigated.

AB - DNA melting temperatures and renaturation rates have been determined for Me2Et2NBr and a series of RMe3NBr and REt3NBr solvents where R is a linear hydrocarbon chain. The point of independence of DNA melting temperature on base composition has been investigated for each solvent system. Renaturation rates are compared with those found in other concentrated salt solutions. Solvent mixtures which accelerate DNA renaturation have also been investigated.

UR - https://www.scopus.com/pages/publications/0017396387

U2 - 10.1002/bip.1977.360160603

DO - 10.1002/bip.1977.360160603

M3 - Article

C2 - 880349

AN - SCOPUS:0017396387

SN - 0006-3525

VL - 16

SP - 1183

EP - 1199

JO - Biopolymers

JF - Biopolymers

IS - 6

ER -

DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions

Abstract

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