TY - JOUR
T1 - DNA damage and repair capacity in patients with lung cancer
T2 - Prediction of multiple primary tumors
AU - Orlow, Irene
AU - Park, Bernard J.
AU - Mujumdar, Urvi
AU - Patel, Himali
AU - Siu-Lau, Puiki
AU - Clas, Brian A.
AU - Downey, Robert
AU - Flores, Raja
AU - Bains, Manjit
AU - Rizk, Nabil
AU - Dominguez, Gemma
AU - Jani, Jen
AU - Berwick, Marianne
AU - Begg, Colin B.
AU - Kris, Mark G.
AU - Rusch, Valerie W.
PY - 2008
Y1 - 2008
N2 - Purpose: Patients who survive one occurrence of non-small-cell lung cancer (NSCLC) are at higher risk of a second malignancy. Capacity to repair damaged DNA may modulate individual susceptibility to develop lung cancer. Therefore, we evaluated constitutive and induced DNA damage, and repair capacity, in patients with multiple NSCLCs (cases) and compared the results to those obtained in patients with a single NSCLC (controls). Patients and Methods: One hundred eight cases and 99 controls matched by age, sex, and time since diagnosis were studied. DNA damage was assessed on peripheral blood lymphocytes by the comet assay before and after exposing cells to a tobacco-derived carcinogen, using the tail moment and the tail intensity as measures to assess baseline damage, induced damage and repair capacity. Results: Constitutive DNA damage, benzo(a)pyrene diol epoxide-induced damage, and repair after BPDE-induced damage were all significantly higher in cases than in controls. These results were confirmed in regression analyses adjusted for potential confounders. Conclusion: DNA damage as measured by the comet assay is associated with the development of multiple primary tumors in individuals with NSCLC.
AB - Purpose: Patients who survive one occurrence of non-small-cell lung cancer (NSCLC) are at higher risk of a second malignancy. Capacity to repair damaged DNA may modulate individual susceptibility to develop lung cancer. Therefore, we evaluated constitutive and induced DNA damage, and repair capacity, in patients with multiple NSCLCs (cases) and compared the results to those obtained in patients with a single NSCLC (controls). Patients and Methods: One hundred eight cases and 99 controls matched by age, sex, and time since diagnosis were studied. DNA damage was assessed on peripheral blood lymphocytes by the comet assay before and after exposing cells to a tobacco-derived carcinogen, using the tail moment and the tail intensity as measures to assess baseline damage, induced damage and repair capacity. Results: Constitutive DNA damage, benzo(a)pyrene diol epoxide-induced damage, and repair after BPDE-induced damage were all significantly higher in cases than in controls. These results were confirmed in regression analyses adjusted for potential confounders. Conclusion: DNA damage as measured by the comet assay is associated with the development of multiple primary tumors in individuals with NSCLC.
UR - https://www.scopus.com/pages/publications/49049110963
U2 - 10.1200/JCO.2007.13.2654
DO - 10.1200/JCO.2007.13.2654
M3 - Article
C2 - 18640936
AN - SCOPUS:49049110963
SN - 0732-183X
VL - 26
SP - 3560
EP - 3566
JO - Journal of Clinical Oncology
JF - Journal of Clinical Oncology
IS - 21
ER -