TY - JOUR
T1 - DNA copy gains of tumor-related genes in vestibular schwannoma
AU - Lassaletta, Luis
AU - Torres-Martín, Miguel
AU - San-Román-Montero, Jesús
AU - Castresana, Javier S.
AU - Gavilán, Javier
AU - Rey, Juan Antonio
PY - 2013/9
Y1 - 2013/9
N2 - DNA copy gains are a common event in tumor growth. This study determines the gene dosage/amplification of seven tumor-related genes in patients undergoing vestibular schwannoma (VS) surgery and analyzes its clinical implications. Thirty-three patients undergoing surgery for VS were studied. Seven genes (EGFR, ERBB2, ERBB3, ERBB4, MDM2, MDM4, and NMYC) were analyzed by Quantitative real-time PCR. Copy gains were correlated with demographic, clinical and radiological data. Of the 33 samples, 48 % were positive for copy gains in at least one gene. There were no positive samples for gene amplification. A clinical correlation between tumor size and copy gains of ERBB2 was found. Patients with copy gains of this gene had larger tumors measured by diameter (p = 0.027) and volume (p = 0.005). Copy gains of EGFR, ERBB2, ERBB4, and MDM4 were associated with preoperative tinnitus. Contrary to other tumors of the central nervous system, development of VS does not appear to involve gene amplification. However, copy gains of certain tumor-related genes may play a role in the biological behavior of these neoplasms. Our findings support the role of ERBB2 in VS development and growth
AB - DNA copy gains are a common event in tumor growth. This study determines the gene dosage/amplification of seven tumor-related genes in patients undergoing vestibular schwannoma (VS) surgery and analyzes its clinical implications. Thirty-three patients undergoing surgery for VS were studied. Seven genes (EGFR, ERBB2, ERBB3, ERBB4, MDM2, MDM4, and NMYC) were analyzed by Quantitative real-time PCR. Copy gains were correlated with demographic, clinical and radiological data. Of the 33 samples, 48 % were positive for copy gains in at least one gene. There were no positive samples for gene amplification. A clinical correlation between tumor size and copy gains of ERBB2 was found. Patients with copy gains of this gene had larger tumors measured by diameter (p = 0.027) and volume (p = 0.005). Copy gains of EGFR, ERBB2, ERBB4, and MDM4 were associated with preoperative tinnitus. Contrary to other tumors of the central nervous system, development of VS does not appear to involve gene amplification. However, copy gains of certain tumor-related genes may play a role in the biological behavior of these neoplasms. Our findings support the role of ERBB2 in VS development and growth
KW - Acoustic neuroma
KW - Amplification
KW - DNA copy gains
KW - Gene copies
KW - Quantitative real-time PCR
KW - Tinnitus
KW - Tumor size
KW - Vestibular schwannoma
UR - http://www.scopus.com/inward/record.url?scp=84881311828&partnerID=8YFLogxK
U2 - 10.1007/s00405-012-2269-z
DO - 10.1007/s00405-012-2269-z
M3 - Article
C2 - 23179934
AN - SCOPUS:84881311828
SN - 0937-4477
VL - 270
SP - 2433
EP - 2438
JO - European Archives of Oto-Rhino-Laryngology
JF - European Archives of Oto-Rhino-Laryngology
IS - 9
ER -