TY - JOUR
T1 - DNA adduct formation among workers in a Thai industrial estate and nearby residents
AU - Peluso, Marco
AU - Srivatanakul, Petcharin
AU - Munnia, Armelle
AU - Jedpiyawongse, Adisorn
AU - Meunier, Aurelie
AU - Sangrajrang, Suleeporn
AU - Piro, Sara
AU - Ceppi, Marcello
AU - Boffetta, Paolo
N1 - Funding Information:
We thank Drs. Yuwadee Chompituk, Somyos Deerasamee, Kris Palasuth, Mrs. Kasemsri Huamchuen and the staff of Rayong Provincial Health Office for their assistance and Prof. Annibale Biggeri for valuable discussions. This work was supported by the budget of National Cancer Institute, Bangkok, Thailand and in part by the “Associazione Italiana per la Ricerca sul Cancro”, Milan, Italy.
PY - 2008/1/25
Y1 - 2008/1/25
N2 - The genotoxic effects of air pollutant exposures have been studied in people living and working in Map Ta Phut, Rayong province, Thailand, a site where is located the Map Ta Phut Industrial Estate (MIE) one of the largest steel, refinery and petrochemical complex in the South-Eastern Asia. This was done by the conduction of a transversal study aimed to compare the prevalence of bulky DNA adducts in groups of subjects experiencing various degree of air pollution. DNA adduct analysis was performed in the leukocytes of 201 volunteers by the 32P-postlabelling assay: 79 were workers in the MIE complex, including 24 refinery workers, 40 steel workers and 15 tinplate workers, 72 were people residing downwind in the MIE area and 50 were residents in a control district of the same Rayong province but without industrial exposures. The groups of workers were analyzed separately to evaluate if DNA adduct formation differs by the type of industry. The levels of bulky DNA adducts were 1.17 ± 0.17 (SE) adducts/108 nucleotides in refinery workers, 1.19 ± 0.19 (SE) in steel workers, 0.87 ± 0.17 (SE) in tinplate workers, 0.85 ± 0.07 (SE) in MIE residents and 0.53 ± 0.05 (SE) in district controls. No effects of smoking habits on DNA adducts was found. The multivariate regression analysis shows that the levels of DNA adducts were significantly increased among the individuals living near the MIE industrial complex in respect to those resident in a control district (p < 0.05). In the groups of occupationally exposed workers, the highest levels of DNA adducts were found among the workers experiencing an occupational exposure to polycyclic aromatic hydrocarbons, e.g. the steel factory and refinery workers. When we have evaluated if the levels of DNA adducts of the PAH exposed workers were different from those of the MIE residents, a statistical significantly difference was found (p < 0.05). Our present study indicates that people living near point sources of industrial air pollution can experiment an excess of DNA adduct formation. The emissions from the MIE complex are the main source of air pollution in this area and can be the cause of such increment in the levels of DNA damage.
AB - The genotoxic effects of air pollutant exposures have been studied in people living and working in Map Ta Phut, Rayong province, Thailand, a site where is located the Map Ta Phut Industrial Estate (MIE) one of the largest steel, refinery and petrochemical complex in the South-Eastern Asia. This was done by the conduction of a transversal study aimed to compare the prevalence of bulky DNA adducts in groups of subjects experiencing various degree of air pollution. DNA adduct analysis was performed in the leukocytes of 201 volunteers by the 32P-postlabelling assay: 79 were workers in the MIE complex, including 24 refinery workers, 40 steel workers and 15 tinplate workers, 72 were people residing downwind in the MIE area and 50 were residents in a control district of the same Rayong province but without industrial exposures. The groups of workers were analyzed separately to evaluate if DNA adduct formation differs by the type of industry. The levels of bulky DNA adducts were 1.17 ± 0.17 (SE) adducts/108 nucleotides in refinery workers, 1.19 ± 0.19 (SE) in steel workers, 0.87 ± 0.17 (SE) in tinplate workers, 0.85 ± 0.07 (SE) in MIE residents and 0.53 ± 0.05 (SE) in district controls. No effects of smoking habits on DNA adducts was found. The multivariate regression analysis shows that the levels of DNA adducts were significantly increased among the individuals living near the MIE industrial complex in respect to those resident in a control district (p < 0.05). In the groups of occupationally exposed workers, the highest levels of DNA adducts were found among the workers experiencing an occupational exposure to polycyclic aromatic hydrocarbons, e.g. the steel factory and refinery workers. When we have evaluated if the levels of DNA adducts of the PAH exposed workers were different from those of the MIE residents, a statistical significantly difference was found (p < 0.05). Our present study indicates that people living near point sources of industrial air pollution can experiment an excess of DNA adduct formation. The emissions from the MIE complex are the main source of air pollution in this area and can be the cause of such increment in the levels of DNA damage.
KW - Air pollution
KW - Bulky DNA adducts
KW - Industrialized area
KW - Map Tha Put
KW - P-postlabelling assay
UR - http://www.scopus.com/inward/record.url?scp=36148990266&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2007.09.012
DO - 10.1016/j.scitotenv.2007.09.012
M3 - Article
C2 - 17935758
AN - SCOPUS:36148990266
SN - 0048-9697
VL - 389
SP - 283
EP - 288
JO - Science of the Total Environment
JF - Science of the Total Environment
IS - 2-3
ER -