Diverse effects of anti-CD44 antibodies on the stromal cell-mediated support of normal but not leukaemic (CML) haemopoiesis in vitro

Saghi Ghaffari, Graeme J. Dougherty, Allen C. Eaves, Connie J. Eaves

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35 Scopus citations

Abstract

We have identified three non-cross-reacting antihuman CD44 monoclonal antibodies that have significant positive or negative (or no) effects on normal human haemopoiesis in the long-term culture (LTC) system. These effects manifested as increases or decreases in the number of LTC-initiating cells (LTC-IC), and the number of colony-forming cells (CFC) recovered from cultures in which either unseparated or highly purified CD34+CD38- normal marrow cells were placed on pre-established normal marrow feeder layers in the presence or absence of each antibody. The effects seen were rapid and sustained, and dependent on the presence of a preformed feeder layer. Interestingly, the same anti-CD44 antibodies had no effect on the maintenance of leukaemic (Ph+) progenitors (from patients with chronic myeloid leukaemia) when these cells were cultured on preformed feeder layers established from normal marrow. CD44 appears to be part of a mechanism by which stromal elements can regulate primitive normal haemopoietic cells but not their leukaemic (Ph+) counterparts.

Original languageEnglish
Pages (from-to)22-28
Number of pages7
JournalBritish Journal of Haematology
Volume97
Issue number1
DOIs
StatePublished - 1997

Keywords

  • CD44
  • chronic myeloid leukaemia
  • long-term cultures
  • marrow microenvironment

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