Distribution and cellular localization of DARPP-32 mRNA in rat brain

Martin Schalling, Mikael Djurfeldt, Tomas Hökfelt, Michelle Ehrlich, Tatsuya Kurihara, Paul Greengard

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

In situ hybridization histochemistry has been used to determine the regional distribution and cellular localization of DARPP-32 mRNA in the rat brain. Results support the concept that DARPP-32 is present primarily in cells expressing the dopamine D1 subtype receptor, and that DARPP-32 is not synthesized in dopamine-containing cells. Strongly labelled neuronal cell bodies were found in the caudate nucleus, nucleus accumbens, olfactory tubercle, parts of the bed nucleus of the stria terminalis, and the amygdaloid complex. In addition large amounts of DARPP-32 mRNA were visualized in the medial habenula and around the third ventricle, in ependymal cells and tanycytes, and in the cerebellar Purkinje cells. A less pronounced activity was seen in layers II-III and VI throughout the cerebral cortex. The present studies together with previous biochemical and immunocytochemical studies demonstrate that DARPP-32 gene expression is greatest primarily in D1 dopaminoceptive cells, although there are exceptions. In situ hybridization may thus be used to quantitate regulation of DARPP-32 mRNA in discrete brain regions.

Original languageEnglish
Pages (from-to)139-149
Number of pages11
JournalMolecular Brain Research
Volume7
Issue number2
DOIs
StatePublished - Feb 1990
Externally publishedYes

Keywords

  • Basal ganglia
  • Dopamine D-receptor
  • In situ hybridization
  • Phosphoprotein
  • Tanycyte

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