Distinct domains of human CDC5 direct its nuclear import and association with the spliceosome

Liansen Liu, Remo Gräub, Myint Hlaing, Conrad L. Epting, Christoph W. Turck, Xiao Qin Xu, Leanne Zhang, Harold S. Bernstein

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Genetic studies have shown that CDC5 proteins are essential for G2 progression and mitotic entry. CDC5 homologs in yeast and mammals are essential for pre-messenger ribonucleic acid (mRNA) processing. Other gene products also have been shown to play roles in both pre-mRNA splicing and cell cycle regulation, prompting the description of several models to explain the mechanism(s) linking these two processes. In this study we demonstrate that the amino-terminus of human CDC5 directs nuclear import independent of consensus nuclear localization signals or phosphorylation, and that the carboxyl-terminus of human CDC5 preferentially associates with spliceosomal complexes in proximity of RNA transcription during interphase. hCDC5 colocalizes with Sm proteins in a cell cycle- and domain-dependent manner, and several proteins in the human CDC5-associated complex are identified that suggest potential roles for the complex in coupling pre-mRNA splicing to transcriptional activation and protein translation. These results indicate that human CDC5 may function in pre-mRNA processing and cell cycle progression through more than one mechanism.

Original languageEnglish
Pages (from-to)119-131
Number of pages13
JournalCell Biochemistry and Biophysics
Volume39
Issue number2
DOIs
StatePublished - Oct 2003
Externally publishedYes

Keywords

  • Cell cycle
  • Nuclear import
  • Nuclear speckle
  • Phosphorylation
  • Spliceosome
  • pre-mRNA splicing

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