Distinct cross-reactive B-cell responses to live attenuated and inactivated influenza vaccines

Sanae Sasaki, Tyson H. Holmes, Randy A. Albrecht, Adolfo García-Sastre, Cornelia L. Dekker, Xiao Song He, Harry B. Greenberg

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


The immunological bases for the efficacies of the 2 currently licensed influenza vaccines, live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV), are not fully understood. The goal of this study was to identify specific B-cell responses correlated with the known efficacies of these 2 vaccines. Methods. We compared the B-cell and antibody responses after immunization with 2010/2011 IIV or LAIV in young adults, focusing on peripheral plasmablasts 6-8 days after vaccination. Results. The quantities of vaccine-specific plasmablasts and plasmablast-derived polyclonal antibodies (PPAbs) in IIV recipients were significantly higher than those in LAIV recipients. No significant difference was detected in the avidity of vaccine-specific PPAbs between the 2 vaccine groups. Proportionally, LAIV induced a greater vaccinespecific immunoglobulin A plasmablast response, as well as a greater plasmablast response to the conserved influenza nuclear protein, than IIV. The cross-reactive plasmablast response to heterovariant strains, as indicated by the relative levels of cross-reactive plasmablasts and the cross-reactive PPAb binding reactivity, was also greater in the LAIV group. Conclusions. Distinct quantitative and qualitative patterns of plasmablast responses were induced by LAIV and IIV in young adults; a proportionally greater cross-reactive response was induced by LAIV.

Original languageEnglish
Pages (from-to)865-874
Number of pages10
JournalJournal of Infectious Diseases
Issue number6
StatePublished - 15 Sep 2014


  • Antibody
  • B-cell response
  • Influenza vaccine


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