Distance effects during polyprotein processing in the complementation between defective FMDV RNAs

Elena Moreno, Celia Perales

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Passage of foot-and-mouth disease virus (FMDV) in BHK-21 cells resulted in the segmentation of the viral genome into two defective RNAs lacking part of either the L- or the capsid-coding region. The two RNAs are infectious by complementation. Electroporation of L-defective RNA in BHK-21 cells resulted in the accumulation of the precursor P3 located away from the deleted sequence. Expression of L in trans led to the processing of P3, indicating that there is a connection between L protease activity and the secondary cleavages carried out by 3C protease within P3. These results suggest that the complementation mechanism between defective RNAs is not restricted to supplying the L and capsid proteins but that distance effects on polyprotein processing events are also implicated.

Original languageEnglish
Pages (from-to)1575-1583
Number of pages9
JournalJournal of General Virology
Volume97
Issue number7
DOIs
StatePublished - Jul 2016
Externally publishedYes

Fingerprint

Dive into the research topics of 'Distance effects during polyprotein processing in the complementation between defective FMDV RNAs'. Together they form a unique fingerprint.

Cite this