Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture

Yulei Wei, E. Zhang, Leqian Yu, Baiquan Ci, Masahiro Sakurai, Lei Guo, Xin Zhang, Sirui Lin, Shino Takii, Lizhong Liu, Jian Liu, Daniel A. Schmitz, Ting Su, Junmei Zhang, Qiaoyan Shen, Yi Ding, Linfeng Zhan, Hai Xi Sun, Canbin Zheng, Lin XuDaiji Okamura, Weizhi Ji, Tao Tan, Jun Wu

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Embryogenesis necessitates harmonious coordination between embryonic and extraembryonic tissues. Although stem cells of both embryonic and extraembryonic origins have been generated, they are grown in different culture conditions. In this study, utilizing a unified culture condition that activates the FGF, TGF-β, and WNT pathways, we have successfully derived embryonic stem cells (FTW-ESCs), extraembryonic endoderm stem cells (FTW-XENs), and trophoblast stem cells (FTW-TSCs) from the three foundational tissues of mouse and cynomolgus monkey (Macaca fascicularis) blastocysts. This approach facilitates the co-culture of embryonic and extraembryonic stem cells, revealing a growth inhibition effect exerted by extraembryonic endoderm cells on pluripotent cells, partially through extracellular matrix signaling. Additionally, our cross-species analysis identified both shared and unique transcription factors and pathways regulating FTW-XENs. The embryonic and extraembryonic stem cell co-culture strategy offers promising avenues for developing more faithful embryo models and devising more developmentally pertinent differentiation protocols.

Original languageEnglish
Pages (from-to)5859-5875.e24
JournalCell
Volume186
Issue number26
DOIs
StatePublished - 21 Dec 2023
Externally publishedYes

Keywords

  • ECM signaling
  • embryonic stem cell
  • extraembryonic endoderm stem cell
  • lineage crosstalk
  • monkey
  • mouse
  • trophoblast stem cell

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