Dispersion and fixation of adeno-associated virus with glutaraldehyde for afm studies

Peng Wang; Xinyan Wang; Haijun Yang; Junhong Lü

doi:10.1142/S0218625X08011688

Dispersion and fixation of adeno-associated virus with glutaraldehyde for afm studies

Peng Wang, Xinyan Wang, Haijun Yang, Junhong Lü

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Sample preparation is an important procedure for atomic force microscope (AFM) studies. However, flexible virus particles have a tendency to aggregate together and are easily compressed during sample preparation or by AFM tip that subsequently hamper studying of virus by AFM. Herein, low concentration chemical reagent of glutaraldehyde (2%, v/v) is pre-mixed in virus suspension that facilitates the dispersion and observation of recombinant serotype 2 adeno-associated virus particles deposited on mica surface with little deformation.

Original language	English
Pages (from-to)	595-598
Number of pages	4
Journal	Surface Review and Letters
Volume	15
Issue number	5
DOIs	https://doi.org/10.1142/S0218625X08011688
State	Published - Oct 2008
Externally published	Yes

Keywords

Adeno-associated virus (AAV)
Atomic force microscope (AFM)
Dispersion
Fixation
Glutaraldehyde

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10.1142/S0218625X08011688

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title = "Dispersion and fixation of adeno-associated virus with glutaraldehyde for afm studies",

abstract = "Sample preparation is an important procedure for atomic force microscope (AFM) studies. However, flexible virus particles have a tendency to aggregate together and are easily compressed during sample preparation or by AFM tip that subsequently hamper studying of virus by AFM. Herein, low concentration chemical reagent of glutaraldehyde (2\%, v/v) is pre-mixed in virus suspension that facilitates the dispersion and observation of recombinant serotype 2 adeno-associated virus particles deposited on mica surface with little deformation.",

keywords = "Adeno-associated virus (AAV), Atomic force microscope (AFM), Dispersion, Fixation, Glutaraldehyde",

author = "Peng Wang and Xinyan Wang and Haijun Yang and Junhong L{\"u}",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (No. 10604061).",

year = "2008",

month = oct,

doi = "10.1142/S0218625X08011688",

language = "English",

volume = "15",

pages = "595--598",

journal = "Surface Review and Letters",

issn = "0218-625X",

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TY - JOUR

T1 - Dispersion and fixation of adeno-associated virus with glutaraldehyde for afm studies

AU - Wang, Peng

AU - Wang, Xinyan

AU - Yang, Haijun

AU - Lü, Junhong

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (No. 10604061).

PY - 2008/10

Y1 - 2008/10

N2 - Sample preparation is an important procedure for atomic force microscope (AFM) studies. However, flexible virus particles have a tendency to aggregate together and are easily compressed during sample preparation or by AFM tip that subsequently hamper studying of virus by AFM. Herein, low concentration chemical reagent of glutaraldehyde (2%, v/v) is pre-mixed in virus suspension that facilitates the dispersion and observation of recombinant serotype 2 adeno-associated virus particles deposited on mica surface with little deformation.

AB - Sample preparation is an important procedure for atomic force microscope (AFM) studies. However, flexible virus particles have a tendency to aggregate together and are easily compressed during sample preparation or by AFM tip that subsequently hamper studying of virus by AFM. Herein, low concentration chemical reagent of glutaraldehyde (2%, v/v) is pre-mixed in virus suspension that facilitates the dispersion and observation of recombinant serotype 2 adeno-associated virus particles deposited on mica surface with little deformation.

KW - Adeno-associated virus (AAV)

KW - Atomic force microscope (AFM)

KW - Dispersion

KW - Fixation

KW - Glutaraldehyde

UR - https://www.scopus.com/pages/publications/55949112753

U2 - 10.1142/S0218625X08011688

DO - 10.1142/S0218625X08011688

M3 - Article

AN - SCOPUS:55949112753

SN - 0218-625X

VL - 15

SP - 595

EP - 598

JO - Surface Review and Letters

JF - Surface Review and Letters

IS - 5

ER -

Dispersion and fixation of adeno-associated virus with glutaraldehyde for afm studies

Abstract

Keywords

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