Disease modifying anti-rheumatic activity of the alkaloid montanine on experimental arthritis and fibroblast-like synoviocytes

Mirian Farinon, Vanessa S. Clarimundo, Graziele P.R. Pedrazza, Pércio S. Gulko, José A.S. Zuanazzi, Ricardo M. Xavier, Patricia G. de Oliveira

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Montanine is an alkaloid isolated from Rhodophiala bifida bulb with potential anti-arthritic activity. In this context, we evaluated whether montanine has a disease modifying anti-rheumatic activity in two arthritis models and its effect in vitro on lymphocyte proliferation and on invasiveness of fibroblast-like synoviocytes (FLS). Antigen-induced arthritis (AIA) was performed in Balb/C mice with methylated bovine serum albumin, and nociception and leukocytes migration into the knee joint were evaluated. Collagen-induced arthritis (CIA) was performed in DBA/1 J mice, and arthritis development and severity were assessed by clinical and histological scoring and articular nociception. Montanine was administered intraperitoneally twice a day. Lymphocyte proliferation stimulated by concanavalin A in 48 h was performed with MTT assay, while FLS invasion in 24 h was assayed in a Matrigel-coated transwell system. Administration of montanine decreased nociception (P<0.001) and leukocyte articular migration (P<0.001) in mice with AIA. In mice with CIA, treatment with montanine reduced severity of arthritis and joint damage assessed by clinical (P<0.001) and histological (P<0.05) scores and ameliorated articular nociception (P<0.05). In vitro, montanine inhibited lymphocyte proliferation stimulated with ConA (P<0.001) and decreased FLS invasion (P<0.05) by 54%, with an action independent of cytotoxicity. Our findings suggest that montanine can be further explored as an innovative pharmacological approach for autoimmune diseases such as arthritis.

Original languageEnglish
Pages (from-to)180-187
Number of pages8
JournalEuropean Journal of Pharmacology
Volume799
DOIs
StatePublished - 2017

Keywords

  • Alkaloids
  • Antigen-induced arthritis
  • Collagen-induced arthritis
  • Experimental arthritis
  • Fibroblast-like synoviocytes
  • Montanine

Fingerprint

Dive into the research topics of 'Disease modifying anti-rheumatic activity of the alkaloid montanine on experimental arthritis and fibroblast-like synoviocytes'. Together they form a unique fingerprint.

Cite this