Direct detection of unexpected alloantibodies in the serum of prospective transfusion recipients using a new hemagglutination inhibition assay

Mathew R. Pincus

Research output: Contribution to journalArticlepeer-review

Abstract

Human red blood cells with antigenic determinants recognized by unexpected alloantibodies are mixed with serum from the potential recipient. Following incubation, multivalent monoclonal IgM antibodies specific to the antigenic determinant present on the test red blood cells are added. If the recipient's serum is negative for an unexpected antibody which would bind to the antigens present on the test red blood cells, then the multivalent antibody will agglutinate the red cells. Conversely, if an unexpected antibody specific to those determinants is present in the recipient's serum, hemagglutination will be inhibited.

Original languageEnglish
Pages (from-to)693
Number of pages1
JournalBiotechnology Advances
Volume15
Issue number3-4
DOIs
StatePublished - 1997
Externally publishedYes

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