Differential hypothalamic neuronal activation following peripheral injection of GLP-1 and oxyntomodulin in mice detected by manganese-enhanced magnetic resonance imaging

Owais B. Chaudhri; James R.C. Parkinson; Yu Ting Kuo; Maralyn R. Druce; Amy H. Herlihy; Jimmy D. Bell; Waljit S. Dhillo; Sarah A. Stanley; Mohammad A. Ghatei; Stephen R. Bloom

doi:10.1016/j.bbrc.2006.09.033

Differential hypothalamic neuronal activation following peripheral injection of GLP-1 and oxyntomodulin in mice detected by manganese-enhanced magnetic resonance imaging

Owais B. Chaudhri, James R.C. Parkinson, Yu Ting Kuo, Maralyn R. Druce, Amy H. Herlihy, Jimmy D. Bell, Waljit S. Dhillo, Sarah A. Stanley, Mohammad A. Ghatei, Stephen R. Bloom

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

The anorexigenic gut hormones oxyntomodulin (OXM) and glucagon-like peptide-1 (GLP-1) are thought to physiologically regulate appetite and food intake. Using manganese-enhanced magnetic resonance imaging, we have shown distinct patterns of neuronal activation in the hypothalamus in response to intraperitoneal injections into fasted mice of 900 and 5400 nmol/kg OXM or 900 nmol/kg GLP-1. Administration of OXM at either dose resulted in a reduced rate of signal enhancement, reflecting a reduction in neuronal activity, in the arcuate, paraventricular, and supraoptic nuclei of the hypothalamus. Conversely, GLP-1 caused a reduction in signal enhancement in the paraventricular nucleus only and an increase in the ventromedial hypothalamic nucleus. Our data show that these two apparently similar peptides generate distinct patterns of activation within the hypothalamus, suggesting that GLP-1 and OXM may act via different hypothalamic pathways.

Original language	English
Pages (from-to)	298-306
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	350
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2006.09.033
State	Published - 17 Nov 2006
Externally published	Yes

Keywords

Appetite
Glucagon-like peptide-1
Gut peptides
Hypothalamus
MRI
Manganese
Mn
Obesity
Oxyntomodulin
Proglucagon

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10.1016/j.bbrc.2006.09.033

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Chaudhri, O. B., Parkinson, J. R. C., Kuo, Y. T., Druce, M. R., Herlihy, A. H., Bell, J. D., Dhillo, W. S., Stanley, S. A., Ghatei, M. A., & Bloom, S. R. (2006). Differential hypothalamic neuronal activation following peripheral injection of GLP-1 and oxyntomodulin in mice detected by manganese-enhanced magnetic resonance imaging. Biochemical and Biophysical Research Communications, 350(2), 298-306. https://doi.org/10.1016/j.bbrc.2006.09.033

@article{4e84604b981d4dd1b6ad0c54b8053967,

title = "Differential hypothalamic neuronal activation following peripheral injection of GLP-1 and oxyntomodulin in mice detected by manganese-enhanced magnetic resonance imaging",

abstract = "The anorexigenic gut hormones oxyntomodulin (OXM) and glucagon-like peptide-1 (GLP-1) are thought to physiologically regulate appetite and food intake. Using manganese-enhanced magnetic resonance imaging, we have shown distinct patterns of neuronal activation in the hypothalamus in response to intraperitoneal injections into fasted mice of 900 and 5400 nmol/kg OXM or 900 nmol/kg GLP-1. Administration of OXM at either dose resulted in a reduced rate of signal enhancement, reflecting a reduction in neuronal activity, in the arcuate, paraventricular, and supraoptic nuclei of the hypothalamus. Conversely, GLP-1 caused a reduction in signal enhancement in the paraventricular nucleus only and an increase in the ventromedial hypothalamic nucleus. Our data show that these two apparently similar peptides generate distinct patterns of activation within the hypothalamus, suggesting that GLP-1 and OXM may act via different hypothalamic pathways.",

keywords = "Appetite, Glucagon-like peptide-1, Gut peptides, Hypothalamus, MRI, Manganese, Mn, Obesity, Oxyntomodulin, Proglucagon",

author = "Chaudhri, {Owais B.} and Parkinson, {James R.C.} and Kuo, {Yu Ting} and Druce, {Maralyn R.} and Herlihy, {Amy H.} and Bell, {Jimmy D.} and Dhillo, {Waljit S.} and Stanley, {Sarah A.} and Ghatei, {Mohammad A.} and Bloom, {Stephen R.}",

note = "Funding Information: The authors are grateful to the Medical Research Council (J.R.C.P.), the Biotechnology and Biological Sciences Research Council (Y.-T.K.), the Wellcome Trust (O.B.C.), and the Department of Health (W.S.D.) for their financial support and to the Statistical Advisory Service of Imperial College London for statistical advice. The authors would also like to thank the Biological Imaging Centre, Imaging Sciences Department, Imperial College London, for the use of the MRI facility and also Violeta Lopez Diaz por inspiratio . ",

year = "2006",

month = nov,

day = "17",

doi = "10.1016/j.bbrc.2006.09.033",

language = "English",

volume = "350",

pages = "298--306",

journal = "Biochemical and Biophysical Research Communications",

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publisher = "Elsevier B.V.",

number = "2",

}

Chaudhri, OB, Parkinson, JRC, Kuo, YT, Druce, MR, Herlihy, AH, Bell, JD, Dhillo, WS, Stanley, SA, Ghatei, MA & Bloom, SR 2006, 'Differential hypothalamic neuronal activation following peripheral injection of GLP-1 and oxyntomodulin in mice detected by manganese-enhanced magnetic resonance imaging', Biochemical and Biophysical Research Communications, vol. 350, no. 2, pp. 298-306. https://doi.org/10.1016/j.bbrc.2006.09.033

Differential hypothalamic neuronal activation following peripheral injection of GLP-1 and oxyntomodulin in mice detected by manganese-enhanced magnetic resonance imaging. / Chaudhri, Owais B.; Parkinson, James R.C.; Kuo, Yu Ting et al.
In: Biochemical and Biophysical Research Communications, Vol. 350, No. 2, 17.11.2006, p. 298-306.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Differential hypothalamic neuronal activation following peripheral injection of GLP-1 and oxyntomodulin in mice detected by manganese-enhanced magnetic resonance imaging

AU - Chaudhri, Owais B.

AU - Parkinson, James R.C.

AU - Kuo, Yu Ting

AU - Druce, Maralyn R.

AU - Herlihy, Amy H.

AU - Bell, Jimmy D.

AU - Dhillo, Waljit S.

AU - Stanley, Sarah A.

AU - Ghatei, Mohammad A.

AU - Bloom, Stephen R.

N1 - Funding Information: The authors are grateful to the Medical Research Council (J.R.C.P.), the Biotechnology and Biological Sciences Research Council (Y.-T.K.), the Wellcome Trust (O.B.C.), and the Department of Health (W.S.D.) for their financial support and to the Statistical Advisory Service of Imperial College London for statistical advice. The authors would also like to thank the Biological Imaging Centre, Imaging Sciences Department, Imperial College London, for the use of the MRI facility and also Violeta Lopez Diaz por inspiratio .

PY - 2006/11/17

Y1 - 2006/11/17

N2 - The anorexigenic gut hormones oxyntomodulin (OXM) and glucagon-like peptide-1 (GLP-1) are thought to physiologically regulate appetite and food intake. Using manganese-enhanced magnetic resonance imaging, we have shown distinct patterns of neuronal activation in the hypothalamus in response to intraperitoneal injections into fasted mice of 900 and 5400 nmol/kg OXM or 900 nmol/kg GLP-1. Administration of OXM at either dose resulted in a reduced rate of signal enhancement, reflecting a reduction in neuronal activity, in the arcuate, paraventricular, and supraoptic nuclei of the hypothalamus. Conversely, GLP-1 caused a reduction in signal enhancement in the paraventricular nucleus only and an increase in the ventromedial hypothalamic nucleus. Our data show that these two apparently similar peptides generate distinct patterns of activation within the hypothalamus, suggesting that GLP-1 and OXM may act via different hypothalamic pathways.

AB - The anorexigenic gut hormones oxyntomodulin (OXM) and glucagon-like peptide-1 (GLP-1) are thought to physiologically regulate appetite and food intake. Using manganese-enhanced magnetic resonance imaging, we have shown distinct patterns of neuronal activation in the hypothalamus in response to intraperitoneal injections into fasted mice of 900 and 5400 nmol/kg OXM or 900 nmol/kg GLP-1. Administration of OXM at either dose resulted in a reduced rate of signal enhancement, reflecting a reduction in neuronal activity, in the arcuate, paraventricular, and supraoptic nuclei of the hypothalamus. Conversely, GLP-1 caused a reduction in signal enhancement in the paraventricular nucleus only and an increase in the ventromedial hypothalamic nucleus. Our data show that these two apparently similar peptides generate distinct patterns of activation within the hypothalamus, suggesting that GLP-1 and OXM may act via different hypothalamic pathways.

KW - Appetite

KW - Glucagon-like peptide-1

KW - Gut peptides

KW - Hypothalamus

KW - MRI

KW - Manganese

KW - Mn

KW - Obesity

KW - Oxyntomodulin

KW - Proglucagon

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U2 - 10.1016/j.bbrc.2006.09.033

DO - 10.1016/j.bbrc.2006.09.033

M3 - Article

C2 - 17007819

AN - SCOPUS:33749478768

SN - 0006-291X

VL - 350

SP - 298

EP - 306

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Differential hypothalamic neuronal activation following peripheral injection of GLP-1 and oxyntomodulin in mice detected by manganese-enhanced magnetic resonance imaging

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Keywords

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