TY - JOUR
T1 - Differential expression of IGF-I and insulin receptor lsoforms in HPV positive and negative human cervical cancer cell lines
AU - Serrano, M. L.
AU - Sánchez-Gómez, M.
AU - Bravo, M. M.
AU - Yakar, S.
AU - LeRoith, D.
PY - 2008/10
Y1 - 2008/10
N2 - Human papillomavirus (HPV) is the main risk factor for cervical cancer; however, some carcinomas occur in the absence of the virus. IGF-IR and an isoform of the insulin receptor, IR-A, play important roles in cancer. In this study we assessed the role of the IGF/insulin receptors in cervical cancer cell lines with different HPV status, SiHa (HPV positive), and C33a (HPV negative). Different patterns of receptor expression were found; while SiHa expressed IGF-IR, IR-A and IR-B, and IR/IGF-IR hybrid receptors, C33a cells expressed the IR-A only. Tyrosine phosphorylation of these receptors in response to their corresponding ligands correlated with the expression level of these receptors in the cell lines. Activation of PI3-K and MAPK pathways was revealed in both cell lines, however, no effects on proliferation, migration, or invasion were observed. Here we show that cervical cancer cell lines - positive and negative for HPV - differ in the type of insulin and IGF-1 receptors expressed. Additional studies are needed for characterization of the role of IR-A in cervical carcinogenesis.
AB - Human papillomavirus (HPV) is the main risk factor for cervical cancer; however, some carcinomas occur in the absence of the virus. IGF-IR and an isoform of the insulin receptor, IR-A, play important roles in cancer. In this study we assessed the role of the IGF/insulin receptors in cervical cancer cell lines with different HPV status, SiHa (HPV positive), and C33a (HPV negative). Different patterns of receptor expression were found; while SiHa expressed IGF-IR, IR-A and IR-B, and IR/IGF-IR hybrid receptors, C33a cells expressed the IR-A only. Tyrosine phosphorylation of these receptors in response to their corresponding ligands correlated with the expression level of these receptors in the cell lines. Activation of PI3-K and MAPK pathways was revealed in both cell lines, however, no effects on proliferation, migration, or invasion were observed. Here we show that cervical cancer cell lines - positive and negative for HPV - differ in the type of insulin and IGF-1 receptors expressed. Additional studies are needed for characterization of the role of IR-A in cervical carcinogenesis.
KW - C33a
KW - Human papillomavirus (HPV)
KW - IR-A
KW - Insulin receptor (IR)
KW - Insulin-like growth factor-I receptor (IGF-IR)
KW - SiHa
UR - http://www.scopus.com/inward/record.url?scp=54049110022&partnerID=8YFLogxK
U2 - 10.1055/s-0028-1082080
DO - 10.1055/s-0028-1082080
M3 - Article
C2 - 18711691
AN - SCOPUS:54049110022
SN - 0018-5043
VL - 40
SP - 661
EP - 667
JO - Hormone and Metabolic Research
JF - Hormone and Metabolic Research
IS - 10
ER -