Differential expression of BDNF mRNA splice variants in mouse brain and immune cells

Niels Kruse; Seray Cetin; Andrew Chan; Ralf Gold; Fred Lühder

doi:10.1016/j.jneuroim.2006.09.001

Differential expression of BDNF mRNA splice variants in mouse brain and immune cells

Niels Kruse, Seray Cetin, Andrew Chan, Ralf Gold, Fred Lühder

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

For the neurotrophin BDNF several splice variants were recently described. We analyzed the expression of mBDNF mRNA splice variants in cells of the immune system in comparison to the central nervous system (CNS). Whereas all splice variants are expressed in the CNS, only mBDNF 3 mRNA could be detected in primary and secondary lymphoid organs as well as in purified T cells and macrophages. After activation, only mBDNF 3 mRNA expression was upregulated in T cells without the additional appearance of other mBDNF splice variants. Therefore, mBDNF mRNA is differentially regulated in the CNS and the immune system opening the possibility of specific manipulation of mBDNF expression in immune cells without globally affecting mBDNF in the CNS.

Original language	English
Pages (from-to)	13-21
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	182
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2006.09.001
State	Published - Jan 2007
Externally published	Yes

Keywords

Brain-derived neurotrophic factor
Macrophages
Neuroimmunology
Neurotrophins
Splice variants
T cells

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10.1016/j.jneuroim.2006.09.001

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@article{d0388a07dff14f0ea15b1656b2cbcced,

title = "Differential expression of BDNF mRNA splice variants in mouse brain and immune cells",

abstract = "For the neurotrophin BDNF several splice variants were recently described. We analyzed the expression of mBDNF mRNA splice variants in cells of the immune system in comparison to the central nervous system (CNS). Whereas all splice variants are expressed in the CNS, only mBDNF 3 mRNA could be detected in primary and secondary lymphoid organs as well as in purified T cells and macrophages. After activation, only mBDNF 3 mRNA expression was upregulated in T cells without the additional appearance of other mBDNF splice variants. Therefore, mBDNF mRNA is differentially regulated in the CNS and the immune system opening the possibility of specific manipulation of mBDNF expression in immune cells without globally affecting mBDNF in the CNS.",

keywords = "Brain-derived neurotrophic factor, Macrophages, Neuroimmunology, Neurotrophins, Splice variants, T cells",

author = "Niels Kruse and Seray Cetin and Andrew Chan and Ralf Gold and Fred L{\"u}hder",

note = "Funding Information: The excellent technical assistance of M. Weig and A. Horn is greatly acknowledged. Additionally we would like to thank Ms. K. Kubera for her help with the mouse breeding and Drs. R. Linker and N.v.Ahsen for critically reading the manuscript. The work was supported by the Fritz und Hildegard Berg Stiftung (T 133-15284) and by the Gemeinn{\"u}tzige Hertie-Stiftung (Grant Nr. 1.01.1/05/009).",

year = "2007",

month = jan,

doi = "10.1016/j.jneuroim.2006.09.001",

language = "English",

volume = "182",

pages = "13--21",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Differential expression of BDNF mRNA splice variants in mouse brain and immune cells

AU - Kruse, Niels

AU - Cetin, Seray

AU - Chan, Andrew

AU - Gold, Ralf

AU - Lühder, Fred

N1 - Funding Information: The excellent technical assistance of M. Weig and A. Horn is greatly acknowledged. Additionally we would like to thank Ms. K. Kubera for her help with the mouse breeding and Drs. R. Linker and N.v.Ahsen for critically reading the manuscript. The work was supported by the Fritz und Hildegard Berg Stiftung (T 133-15284) and by the Gemeinnützige Hertie-Stiftung (Grant Nr. 1.01.1/05/009).

PY - 2007/1

Y1 - 2007/1

N2 - For the neurotrophin BDNF several splice variants were recently described. We analyzed the expression of mBDNF mRNA splice variants in cells of the immune system in comparison to the central nervous system (CNS). Whereas all splice variants are expressed in the CNS, only mBDNF 3 mRNA could be detected in primary and secondary lymphoid organs as well as in purified T cells and macrophages. After activation, only mBDNF 3 mRNA expression was upregulated in T cells without the additional appearance of other mBDNF splice variants. Therefore, mBDNF mRNA is differentially regulated in the CNS and the immune system opening the possibility of specific manipulation of mBDNF expression in immune cells without globally affecting mBDNF in the CNS.

AB - For the neurotrophin BDNF several splice variants were recently described. We analyzed the expression of mBDNF mRNA splice variants in cells of the immune system in comparison to the central nervous system (CNS). Whereas all splice variants are expressed in the CNS, only mBDNF 3 mRNA could be detected in primary and secondary lymphoid organs as well as in purified T cells and macrophages. After activation, only mBDNF 3 mRNA expression was upregulated in T cells without the additional appearance of other mBDNF splice variants. Therefore, mBDNF mRNA is differentially regulated in the CNS and the immune system opening the possibility of specific manipulation of mBDNF expression in immune cells without globally affecting mBDNF in the CNS.

KW - Brain-derived neurotrophic factor

KW - Macrophages

KW - Neuroimmunology

KW - Neurotrophins

KW - Splice variants

KW - T cells

UR - http://www.scopus.com/inward/record.url?scp=33845878791&partnerID=8YFLogxK

U2 - 10.1016/j.jneuroim.2006.09.001

DO - 10.1016/j.jneuroim.2006.09.001

M3 - Article

C2 - 17046071

AN - SCOPUS:33845878791

SN - 0165-5728

VL - 182

SP - 13

EP - 21

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

Differential expression of BDNF mRNA splice variants in mouse brain and immune cells

Abstract

Keywords

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