Differences in protein patterns of influenza A viruses

Mary B. Ritchey; Peter Palese; Jerome L. Schulman

doi:10.1016/0042-6822(77)90289-6

Differences in protein patterns of influenza A viruses

Mary B. Ritchey
, Peter Palese
, Jerome L. Schulman

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Analysis of virus-specific proteins of two influenza A viruses on SDS gradient polyacrylamide gels suggests the presence of a previously unrecognized third P protein, P3, which indicates that the influenza virus genome codes for a total of eight proteins. In addition, it was demonstrated that the migration of all proteins of influenza A/PR/34 (H0N1) virus (except neuraminidase) can be distinguished from the corresponding proteins of influenza A/Hong Kong/8/68 (H3N2) virus in this gel system. Thus, variations previously observed in the migration patterns of the RNAs of these two strains are reflected by significant differences in all gene products. It is also possible to demonstrate the presence of a precursor hemagglutinin in this system. In addition, this system provides a convenient method for characterization of influenza virus recombinants with respect to the derivation of genes coding for nonsurface proteins.

Original language	English
Pages (from-to)	122-128
Number of pages	7
Journal	Virology
Volume	76
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(77)90289-6
State	Published - Jan 1977
Externally published	Yes

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@article{21beecb9cdac4219a993b275a1213456,

title = "Differences in protein patterns of influenza A viruses",

abstract = "Analysis of virus-specific proteins of two influenza A viruses on SDS gradient polyacrylamide gels suggests the presence of a previously unrecognized third P protein, P3, which indicates that the influenza virus genome codes for a total of eight proteins. In addition, it was demonstrated that the migration of all proteins of influenza A/PR/34 (H0N1) virus (except neuraminidase) can be distinguished from the corresponding proteins of influenza A/Hong Kong/8/68 (H3N2) virus in this gel system. Thus, variations previously observed in the migration patterns of the RNAs of these two strains are reflected by significant differences in all gene products. It is also possible to demonstrate the presence of a precursor hemagglutinin in this system. In addition, this system provides a convenient method for characterization of influenza virus recombinants with respect to the derivation of genes coding for nonsurface proteins.",

author = "Ritchey, \{Mary B.\} and Peter Palese and Schulman, \{Jerome L.\}",

note = "Funding Information: ACKNOWLEDGMENTS The authors wish to thank Ms. Kaye Leitzinger for expert This work was made possible National Institutes of Health 09394) and from the National (PCM-76-1106). M. B. Ritchey training grant (I-TOI-AI-00439) Institutes of Health.",

year = "1977",

month = jan,

doi = "10.1016/0042-6822(77)90289-6",

language = "English",

volume = "76",

pages = "122--128",

journal = "Virology",

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publisher = "Academic Press Inc.",

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T1 - Differences in protein patterns of influenza A viruses

AU - Ritchey, Mary B.

AU - Palese, Peter

AU - Schulman, Jerome L.

N1 - Funding Information: ACKNOWLEDGMENTS The authors wish to thank Ms. Kaye Leitzinger for expert This work was made possible National Institutes of Health 09394) and from the National (PCM-76-1106). M. B. Ritchey training grant (I-TOI-AI-00439) Institutes of Health.

PY - 1977/1

Y1 - 1977/1

N2 - Analysis of virus-specific proteins of two influenza A viruses on SDS gradient polyacrylamide gels suggests the presence of a previously unrecognized third P protein, P3, which indicates that the influenza virus genome codes for a total of eight proteins. In addition, it was demonstrated that the migration of all proteins of influenza A/PR/34 (H0N1) virus (except neuraminidase) can be distinguished from the corresponding proteins of influenza A/Hong Kong/8/68 (H3N2) virus in this gel system. Thus, variations previously observed in the migration patterns of the RNAs of these two strains are reflected by significant differences in all gene products. It is also possible to demonstrate the presence of a precursor hemagglutinin in this system. In addition, this system provides a convenient method for characterization of influenza virus recombinants with respect to the derivation of genes coding for nonsurface proteins.

AB - Analysis of virus-specific proteins of two influenza A viruses on SDS gradient polyacrylamide gels suggests the presence of a previously unrecognized third P protein, P3, which indicates that the influenza virus genome codes for a total of eight proteins. In addition, it was demonstrated that the migration of all proteins of influenza A/PR/34 (H0N1) virus (except neuraminidase) can be distinguished from the corresponding proteins of influenza A/Hong Kong/8/68 (H3N2) virus in this gel system. Thus, variations previously observed in the migration patterns of the RNAs of these two strains are reflected by significant differences in all gene products. It is also possible to demonstrate the presence of a precursor hemagglutinin in this system. In addition, this system provides a convenient method for characterization of influenza virus recombinants with respect to the derivation of genes coding for nonsurface proteins.

UR - https://www.scopus.com/pages/publications/0017367878

U2 - 10.1016/0042-6822(77)90289-6

DO - 10.1016/0042-6822(77)90289-6

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SN - 0042-6822

VL - 76

SP - 122

EP - 128

JO - Virology

JF - Virology

IS - 1

ER -

Differences in protein patterns of influenza A viruses

Abstract

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