Differences in protein patterns of influenza A viruses

Mary B. Ritchey, Peter Palese, Jerome L. Schulman

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Analysis of virus-specific proteins of two influenza A viruses on SDS gradient polyacrylamide gels suggests the presence of a previously unrecognized third P protein, P3, which indicates that the influenza virus genome codes for a total of eight proteins. In addition, it was demonstrated that the migration of all proteins of influenza A/PR/34 (H0N1) virus (except neuraminidase) can be distinguished from the corresponding proteins of influenza A/Hong Kong/8/68 (H3N2) virus in this gel system. Thus, variations previously observed in the migration patterns of the RNAs of these two strains are reflected by significant differences in all gene products. It is also possible to demonstrate the presence of a precursor hemagglutinin in this system. In addition, this system provides a convenient method for characterization of influenza virus recombinants with respect to the derivation of genes coding for nonsurface proteins.

Original languageEnglish
Pages (from-to)122-128
Number of pages7
JournalVirology
Volume76
Issue number1
DOIs
StatePublished - Jan 1977
Externally publishedYes

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