Differences in chemotherapeutic susceptibility of human T-, B-, and non-T-/non-B-lymphocytes in culture.

T. Ohnuma, H. Arkin, J. F. Holland

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7 Scopus citations

Abstract

Eleven human lymphoid cell lines, two T-cell lines, six B-cell lines and three non-T-/non-B-cell lines were evaluated for their asparagine dependence and for their chemotherapeutic susceptibility to asparaginase, cytosine arabinoside (ara-C), and 5-fluorouracil (FU). Two T-cell lines were asparagine dependent, whereas all B-cell and non-T-/non-B-cell lines were asparagine independent. These differences in nutritional requirements were consistent with as much as 5,000-fold differences in asparaginase sensitivity. B cells were found to be as much as 200-fold less sensitive to ara-C than T cells, irrespective of the benign or malignant nature of the cells or the presence or absence of EB virus infection. One non-T-/non-B-cell line with cell markers similar to the B-cell group behaved like a B-cell line. Two other non-T-/non-B-cells showed unique ara-C dose-response curves. FU sensitivity study revealed heterogeneity among B-cell groups. Non-T-/non-B-cell lines were uniformly FU insensitive. These differences in chemotherapeutic susceptibility were discussed in terms of usefulness as an in vitro model.

Original languageEnglish
Pages (from-to)61-67
Number of pages7
JournalRecent Results in Cancer Research
Volume75
DOIs
StatePublished - 1980

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