Dicer is essential for mouse development

Emily Bernstein, Sang Yong Kim, Michelle A. Carmell, Elizabeth P. Murchison, Heather Alcorn, Mamie Z. Li, Alea A. Mills, Stephen J. Elledge, Kathryn V. Anderson, Gregory J. Hannon

Research output: Contribution to journalArticlepeer-review

1638 Scopus citations

Abstract

To address the biological function of RNA interference (RNAi)-related pathways in mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in development, with Dicer1-null embryos depleted of stem cells. Coupled with our inability to generate viable Dicer1-null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development.

Original languageEnglish
Pages (from-to)215-217
Number of pages3
JournalNature Genetics
Volume35
Issue number3
DOIs
StatePublished - Nov 2003
Externally publishedYes

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