Diagnostic fluorescence spectroscopy of oral mucosa

Krishnendu Roy, Ian D. Bottrill, Duncan R. Ingrams, Mjchajl M. Pankratov, Elie E. Rebeiz, Peak Woo, Sadru Kabani, Stanley M. Shapshay, Ramasamy Manoharan, Lrving Itzkan, Michael S. Feld

Research output: Contribution to journalConference articlepeer-review

22 Scopus citations


Autofluorescence characteristics of normal, dysplastic, and malignant squamous tissues from the oral cavity were measured with a spectrofluorometer in the excitation range of 250-500 nm and emission range of 350-750 nm. Fluorescence excitation-emission matrices (EEM) were obtained from samples collected from patients in the clinic and in the operating room. The same samples were submitted for histopathological examination following spectroscopic measurements. The contour plots obtained from the EEMs of the samples showed consistent differences between normal and abnormal tissues. All the abnormal samples showed enhanced red region (> 600 nm) fluorescence with a prominent peak at 635 nm, when excited by 4 1 0 nm light. A ratio contour plot (abnormal/normal) enhanced spectral differences in the red region. A fiber-optic based spectrofluorometer for EEM measurements is being developed for further investigations.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 12 May 1995
Externally publishedYes
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems V 1995 - San Jose, United States
Duration: 1 Feb 199528 Feb 1995


  • Dysplasia
  • Fluorescence
  • Oral Mucosa
  • Spectroscopy


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