TY - JOUR
T1 - Diagnosis of renal proximal tubular injury by urinary immunoassay for a proximal tubular antigen, the adenosine deaminase binding protein
AU - Tolkoff-Rubin, N. E.
AU - Thompson, R. E.
AU - Piper, D. J.
AU - Hansen, W. P.
AU - Bander, N. H.
AU - Cordon-Cardo, C.
AU - Finstad, C. J.
AU - Klotz, L. H.
AU - Old, L. J.
AU - Rubin, R. H.
PY - 1987
Y1 - 1987
N2 - A sandwich ELISA assay has been formatted from two commercially available murine monoclonal antibodies, URO-4 and URO-4a, directed against a 120 000 dalton glycoprotein, the adenosine deaminase binding protein (ABP), found on the brush border of the renal proximal tubular epithelial cell. Untimed urine samples from 37 normal individuals and urinary ABP <0.1 AU; 37 patients with pure glomerular disease had ABP <0.4 AU (with 29, or 76% <0.2 AU); 10 patients with pre-renal azotaemia had ABP <0.6 (with 8, or 80% <0.3 AU). In contrast, 79 patients with post-ischaemic acute tubular necrosis had ABP >0.6 AU. Acute renal failure due to myoglobinuria, contrast dye, and aminoglycoside toxicity were all associated with urinary ABP >1.0 AU. In addition, all six patients with acute bacteraemic pyelonephritis had ABP >0.7 AU, as opposed to ABP <0.2 AU in the urines of 12 women with acute cystitis. We conclude that this monoclonal antibody based urinary assay is a sensitive measure of renal proximal tubular injury, reliably distinguishes acute tubular from glomerular disease, and may be helpful in differentiating forms of urinary tract infection.
AB - A sandwich ELISA assay has been formatted from two commercially available murine monoclonal antibodies, URO-4 and URO-4a, directed against a 120 000 dalton glycoprotein, the adenosine deaminase binding protein (ABP), found on the brush border of the renal proximal tubular epithelial cell. Untimed urine samples from 37 normal individuals and urinary ABP <0.1 AU; 37 patients with pure glomerular disease had ABP <0.4 AU (with 29, or 76% <0.2 AU); 10 patients with pre-renal azotaemia had ABP <0.6 (with 8, or 80% <0.3 AU). In contrast, 79 patients with post-ischaemic acute tubular necrosis had ABP >0.6 AU. Acute renal failure due to myoglobinuria, contrast dye, and aminoglycoside toxicity were all associated with urinary ABP >1.0 AU. In addition, all six patients with acute bacteraemic pyelonephritis had ABP >0.7 AU, as opposed to ABP <0.2 AU in the urines of 12 women with acute cystitis. We conclude that this monoclonal antibody based urinary assay is a sensitive measure of renal proximal tubular injury, reliably distinguishes acute tubular from glomerular disease, and may be helpful in differentiating forms of urinary tract infection.
UR - http://www.scopus.com/inward/record.url?scp=0023513877&partnerID=8YFLogxK
M3 - Article
C2 - 2888057
AN - SCOPUS:0023513877
SN - 0931-0509
VL - 2
SP - 143
EP - 148
JO - Nephrology Dialysis Transplantation
JF - Nephrology Dialysis Transplantation
IS - 3
ER -