TY - JOUR
T1 - Developmental regulation of phosphoprotein gene expression in the caudate-putamen of rat
T2 - An in situ hybridization study
AU - Gustafson, E. L.
AU - Ehrlich, M. E.
AU - Trivedi, P.
AU - Greengard, P.
N1 - Funding Information:
Acknowledgements-Supporled by USPHS Program Project MH-40899, a NARSAD Young Investigator Award to E.L.G. and USPHS MH-00606 and an American Parkinson Disease Foundation Award to M.E.E. The authors would like to thank Dr Charles Ouimet for advice on the manuscript, and Dr Michael Lewis for advice and assistancew ith the in situ hybridization.
PY - 1992/11
Y1 - 1992/11
N2 - The regional and cellular ontogeny of the mRNA encoding the dopamine- and cAMP-regulated phosphoprotein, DARPP-32, has been studied in rat striatum by quantitative in situ hybridization histochemistry. The mRNA for DARPP-32 exhibited a characteristic developmental profile. The hybridization signal was first visible on the day of birth, at which time DARPP-32 mRNA was concentrated in patches in the caudate-putamen. By the end of the first postnatal week, the majority of neurons in the caudate putamen expressed the DARPP-32 message. Levels of mRNA per cell increased markedly during the second postnatal week and peaked around the beginning of the third week. The adult level of DARPP-32 mRNA was lower than that observed at the apex of mRNA expression, on a per cell basis, while the proportion of neurons expressing detectable levels of message remained relatively constant. In the nucleus accumbens and olfactory tubercle, DARPP-32 mRNA development lagged somewhat behind that observed in the caudate-putamen, but was similar in other respects. A non-quantitative study employing an oligonucleotide probe complementary to the mRNA encoding another cAMP-regulated phosphoprotein, ARPP-21, revealed a similar developmental sequence to DARPP-32. The present results suggest that for DARPP-32 mRNA, genetic and, possibly, environmental factors play a role in determining the developmental patterns observed.
AB - The regional and cellular ontogeny of the mRNA encoding the dopamine- and cAMP-regulated phosphoprotein, DARPP-32, has been studied in rat striatum by quantitative in situ hybridization histochemistry. The mRNA for DARPP-32 exhibited a characteristic developmental profile. The hybridization signal was first visible on the day of birth, at which time DARPP-32 mRNA was concentrated in patches in the caudate-putamen. By the end of the first postnatal week, the majority of neurons in the caudate putamen expressed the DARPP-32 message. Levels of mRNA per cell increased markedly during the second postnatal week and peaked around the beginning of the third week. The adult level of DARPP-32 mRNA was lower than that observed at the apex of mRNA expression, on a per cell basis, while the proportion of neurons expressing detectable levels of message remained relatively constant. In the nucleus accumbens and olfactory tubercle, DARPP-32 mRNA development lagged somewhat behind that observed in the caudate-putamen, but was similar in other respects. A non-quantitative study employing an oligonucleotide probe complementary to the mRNA encoding another cAMP-regulated phosphoprotein, ARPP-21, revealed a similar developmental sequence to DARPP-32. The present results suggest that for DARPP-32 mRNA, genetic and, possibly, environmental factors play a role in determining the developmental patterns observed.
UR - http://www.scopus.com/inward/record.url?scp=0026483305&partnerID=8YFLogxK
U2 - 10.1016/0306-4522(92)90471-D
DO - 10.1016/0306-4522(92)90471-D
M3 - Article
C2 - 1465187
AN - SCOPUS:0026483305
SN - 0306-4522
VL - 51
SP - 65
EP - 75
JO - Neuroscience
JF - Neuroscience
IS - 1
ER -