Development of a novel serological assay for the detection of mpox infection in vaccinated populations

The PVI Study Group

doi:10.1002/jmv.29134

Development of a novel serological assay for the detection of mpox infection in vaccinated populations

The PVI Study Group

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In 2022 the World Health Organization declared a Public Health Emergency for an outbreak of mpox, the zoonotic Orthopoxvirus (OPV) affecting at least 104 nonendemic locations worldwide. Serologic detection of mpox infection is problematic, however, due to considerable antigenic and serologic cross-reactivity among OPVs and smallpox-vaccinated individuals. In this report, we developed a high-throughput multiplex microsphere immunoassay using a combination of mpox-specific peptides and cross-reactive OPV proteins that results in the specific serologic detection of mpox infection with 93% sensitivity and 98% specificity. The New York State Non-Vaccinia Orthopoxvirus Microsphere Immunoassay is an important tool to detect subclinical mpox infection and understand the extent of mpox spread in the community through retrospective analysis.

Original language	English
Article number	e29134
Journal	Journal of Medical Virology
Volume	95
Issue number	10
DOIs	https://doi.org/10.1002/jmv.29134
State	Published - Oct 2023

Keywords

antibody
immunoassay
mpox
orthopoxvirus
serology

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title = "Development of a novel serological assay for the detection of mpox infection in vaccinated populations",

abstract = "In 2022 the World Health Organization declared a Public Health Emergency for an outbreak of mpox, the zoonotic Orthopoxvirus (OPV) affecting at least 104 nonendemic locations worldwide. Serologic detection of mpox infection is problematic, however, due to considerable antigenic and serologic cross-reactivity among OPVs and smallpox-vaccinated individuals. In this report, we developed a high-throughput multiplex microsphere immunoassay using a combination of mpox-specific peptides and cross-reactive OPV proteins that results in the specific serologic detection of mpox infection with 93% sensitivity and 98% specificity. The New York State Non-Vaccinia Orthopoxvirus Microsphere Immunoassay is an important tool to detect subclinical mpox infection and understand the extent of mpox spread in the community through retrospective analysis.",

keywords = "antibody, immunoassay, mpox, orthopoxvirus, serology",

author = "{The PVI Study Group} and Yates, {Jennifer L.} and Hunt, {Danielle T.} and Kulas, {Karen E.} and Chave, {Karen J.} and Linda Styer and Chakravarthi, {Sandhya T.} and Cai, {Gianna Y.} and Berm{\'u}dez-Gonz{\'a}lez, {Maria C.} and Giulio Kleiner and Deena Altman and Komal Srivastava and Viviana Simon and Dennis Feihel and Joseph McGowan and Wayne Hogrefe and Philip Noone and Christina Egan and Slifka, {Mark K.} and Lee, {William T.}",

note = "Publisher Copyright: {\textcopyright} 2023 Wiley Periodicals LLC.",

year = "2023",

month = oct,

doi = "10.1002/jmv.29134",

language = "English",

volume = "95",

journal = "Journal of Medical Virology",

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AU - The PVI Study Group

AU - Yates, Jennifer L.

AU - Hunt, Danielle T.

AU - Kulas, Karen E.

AU - Chave, Karen J.

AU - Styer, Linda

AU - Chakravarthi, Sandhya T.

AU - Cai, Gianna Y.

AU - Bermúdez-González, Maria C.

AU - Kleiner, Giulio

AU - Altman, Deena

AU - Srivastava, Komal

AU - Simon, Viviana

AU - Feihel, Dennis

AU - McGowan, Joseph

AU - Hogrefe, Wayne

AU - Noone, Philip

AU - Egan, Christina

AU - Slifka, Mark K.

AU - Lee, William T.

PY - 2023/10

Y1 - 2023/10

N2 - In 2022 the World Health Organization declared a Public Health Emergency for an outbreak of mpox, the zoonotic Orthopoxvirus (OPV) affecting at least 104 nonendemic locations worldwide. Serologic detection of mpox infection is problematic, however, due to considerable antigenic and serologic cross-reactivity among OPVs and smallpox-vaccinated individuals. In this report, we developed a high-throughput multiplex microsphere immunoassay using a combination of mpox-specific peptides and cross-reactive OPV proteins that results in the specific serologic detection of mpox infection with 93% sensitivity and 98% specificity. The New York State Non-Vaccinia Orthopoxvirus Microsphere Immunoassay is an important tool to detect subclinical mpox infection and understand the extent of mpox spread in the community through retrospective analysis.

AB - In 2022 the World Health Organization declared a Public Health Emergency for an outbreak of mpox, the zoonotic Orthopoxvirus (OPV) affecting at least 104 nonendemic locations worldwide. Serologic detection of mpox infection is problematic, however, due to considerable antigenic and serologic cross-reactivity among OPVs and smallpox-vaccinated individuals. In this report, we developed a high-throughput multiplex microsphere immunoassay using a combination of mpox-specific peptides and cross-reactive OPV proteins that results in the specific serologic detection of mpox infection with 93% sensitivity and 98% specificity. The New York State Non-Vaccinia Orthopoxvirus Microsphere Immunoassay is an important tool to detect subclinical mpox infection and understand the extent of mpox spread in the community through retrospective analysis.

KW - antibody

KW - immunoassay

KW - mpox

KW - orthopoxvirus

KW - serology

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U2 - 10.1002/jmv.29134

DO - 10.1002/jmv.29134

M3 - Article

AN - SCOPUS:85174566127

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VL - 95

JO - Journal of Medical Virology

JF - Journal of Medical Virology

IS - 10

M1 - e29134

ER -

Development of a novel serological assay for the detection of mpox infection in vaccinated populations

Abstract

Keywords

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