Development of a novel immuno-PCR for detection of avian leukosis virus

Quan Xie, Jianjun Zhang, Hongxia Shao, Zhimin Wan, Xiaoyan Tian, Jialiang Yang, Mayun Pang, Kun Qian, Wei Gao, Chengming Wang, Aijian Qin, Jianqiang Ye

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Avian leukosis virus (ALV) is an important pathogen for various neoplasms, including lymphoid, myeloid, and erythroid neoplasms, and it causes significant economic loss in the poultry industry. Several efficient methods for the detection of ALV have been reported. However, these previously developed approaches are based on either PCR or immunoassays. Here, we used a proximity ligation technique and combined PCR with the immunoassay to develop a novel immuno-PCR (Im-PCR) approach for the detection of ALV. Our data showed that the Im-PCR had high specificity and sensitivity to ALV. The Im-PCR method selectively reacted to ALV but not to the other avian viruses tested. The limit of detection of Im-PCR could reach 0.5 TCID50. Moreover, the results of Im-PCR were in agreement with results from commercial ELISA when the clinical cloaca samples were used for ALV detection. The present results demonstrate that the novel Im-PCR method can be efficiently applied to detect ALV in a clinical setting. Our data also highlight that Im-PCR may have promising applications in the diagnosis of pathogens.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalJournal of Virological Methods
Volume236
DOIs
StatePublished - 1 Oct 2016

Keywords

  • Avian leukosis virus
  • Detection
  • Immuno-PCR
  • Proximity ligation assay

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