Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography

M. Fairclough; B. Ellis; H. Boutin; A. K.P. Jones; A. McMahon; S. Alzabin; A. Gennari; C. Prenant

doi:10.1016/j.apradiso.2017.09.004

Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography

M. Fairclough
, B. Ellis
, H. Boutin
, A. K.P. Jones
, A. McMahon
, S. Alzabin
, A. Gennari
, C. Prenant

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (⁸⁹Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210 nm and zeta potential +46 ± 4 mV) as the carrier. We propose that cell uptake of ⁸⁹Zr-loaded CN occurred in a two-step process; cell membrane interaction with ⁸⁹Zr-loaded CN was followed by a slower cell internalisation step.

Original language	English
Pages (from-to)	7-12
Number of pages	6
Journal	Applied Radiation and Isotopes
Volume	130
DOIs	https://doi.org/10.1016/j.apradiso.2017.09.004
State	Published - Dec 2017
Externally published	Yes

Keywords

Chitosan
Inflammation imaging
Leukocyte trafficking
Nanoparticles
PET
Zr-89

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10.1016/j.apradiso.2017.09.004

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Fairclough, M., Ellis, B., Boutin, H., Jones, A. K. P., McMahon, A., Alzabin, S., Gennari, A., & Prenant, C. (2017). Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography. Applied Radiation and Isotopes, 130, 7-12. https://doi.org/10.1016/j.apradiso.2017.09.004

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title = "Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography",

abstract = "Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (89Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210 nm and zeta potential +46 ± 4 mV) as the carrier. We propose that cell uptake of 89Zr-loaded CN occurred in a two-step process; cell membrane interaction with 89Zr-loaded CN was followed by a slower cell internalisation step.",

keywords = "Chitosan, Inflammation imaging, Leukocyte trafficking, Nanoparticles, PET, Zr-89",

author = "M. Fairclough and B. Ellis and H. Boutin and Jones, \{A. K.P.\} and A. McMahon and S. Alzabin and A. Gennari and C. Prenant",

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year = "2017",

month = dec,

doi = "10.1016/j.apradiso.2017.09.004",

language = "English",

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Fairclough, M, Ellis, B, Boutin, H, Jones, AKP, McMahon, A, Alzabin, S, Gennari, A & Prenant, C 2017, 'Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography', Applied Radiation and Isotopes, vol. 130, pp. 7-12. https://doi.org/10.1016/j.apradiso.2017.09.004

TY - JOUR

T1 - Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography

AU - Fairclough, M.

AU - Ellis, B.

AU - Boutin, H.

AU - Jones, A. K.P.

AU - McMahon, A.

AU - Alzabin, S.

AU - Gennari, A.

AU - Prenant, C.

PY - 2017/12

Y1 - 2017/12

N2 - Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (89Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210 nm and zeta potential +46 ± 4 mV) as the carrier. We propose that cell uptake of 89Zr-loaded CN occurred in a two-step process; cell membrane interaction with 89Zr-loaded CN was followed by a slower cell internalisation step.

AB - Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (89Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210 nm and zeta potential +46 ± 4 mV) as the carrier. We propose that cell uptake of 89Zr-loaded CN occurred in a two-step process; cell membrane interaction with 89Zr-loaded CN was followed by a slower cell internalisation step.

KW - Chitosan

KW - Inflammation imaging

KW - Leukocyte trafficking

KW - Nanoparticles

KW - PET

KW - Zr-89

UR - https://www.scopus.com/pages/publications/85029421230

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DO - 10.1016/j.apradiso.2017.09.004

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AN - SCOPUS:85029421230

SN - 0969-8043

VL - 130

SP - 7

EP - 12

JO - Applied Radiation and Isotopes

JF - Applied Radiation and Isotopes

ER -

Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography

Abstract

Keywords

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