Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography

M. Fairclough; B. Ellis; H. Boutin; A. K.P. Jones; A. McMahon; S. Alzabin; A. Gennari; C. Prenant

doi:10.1016/j.apradiso.2017.09.004

Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography

M. Fairclough, B. Ellis, H. Boutin, A. K.P. Jones, A. McMahon, S. Alzabin, A. Gennari, C. Prenant

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (⁸⁹Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210 nm and zeta potential +46 ± 4 mV) as the carrier. We propose that cell uptake of ⁸⁹Zr-loaded CN occurred in a two-step process; cell membrane interaction with ⁸⁹Zr-loaded CN was followed by a slower cell internalisation step.

Original language	English
Pages (from-to)	7-12
Number of pages	6
Journal	Applied Radiation and Isotopes
Volume	130
DOIs	https://doi.org/10.1016/j.apradiso.2017.09.004
State	Published - Dec 2017
Externally published	Yes

Keywords

Chitosan
Inflammation imaging
Leukocyte trafficking
Nanoparticles
PET
Zr-89

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10.1016/j.apradiso.2017.09.004

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Fairclough, M., Ellis, B., Boutin, H., Jones, A. K. P., McMahon, A., Alzabin, S., Gennari, A., & Prenant, C. (2017). Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography. Applied Radiation and Isotopes, 130, 7-12. https://doi.org/10.1016/j.apradiso.2017.09.004

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abstract = "Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (89Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210 nm and zeta potential +46 ± 4 mV) as the carrier. We propose that cell uptake of 89Zr-loaded CN occurred in a two-step process; cell membrane interaction with 89Zr-loaded CN was followed by a slower cell internalisation step.",

keywords = "Chitosan, Inflammation imaging, Leukocyte trafficking, Nanoparticles, PET, Zr-89",

author = "M. Fairclough and B. Ellis and H. Boutin and Jones, {A. K.P.} and A. McMahon and S. Alzabin and A. Gennari and C. Prenant",

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year = "2017",

month = dec,

doi = "10.1016/j.apradiso.2017.09.004",

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Fairclough, M, Ellis, B, Boutin, H, Jones, AKP, McMahon, A, Alzabin, S, Gennari, A & Prenant, C 2017, 'Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography', Applied Radiation and Isotopes, vol. 130, pp. 7-12. https://doi.org/10.1016/j.apradiso.2017.09.004

TY - JOUR

T1 - Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography

AU - Fairclough, M.

AU - Ellis, B.

AU - Boutin, H.

AU - Jones, A. K.P.

AU - McMahon, A.

AU - Alzabin, S.

AU - Gennari, A.

AU - Prenant, C.

PY - 2017/12

Y1 - 2017/12

N2 - Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (89Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210 nm and zeta potential +46 ± 4 mV) as the carrier. We propose that cell uptake of 89Zr-loaded CN occurred in a two-step process; cell membrane interaction with 89Zr-loaded CN was followed by a slower cell internalisation step.

AB - Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (89Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210 nm and zeta potential +46 ± 4 mV) as the carrier. We propose that cell uptake of 89Zr-loaded CN occurred in a two-step process; cell membrane interaction with 89Zr-loaded CN was followed by a slower cell internalisation step.

KW - Chitosan

KW - Inflammation imaging

KW - Leukocyte trafficking

KW - Nanoparticles

KW - PET

KW - Zr-89

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JO - Applied Radiation and Isotopes

JF - Applied Radiation and Isotopes

ER -

Development of a method for the preparation of zirconium-89 radiolabelled chitosan nanoparticles as an application for leukocyte trafficking with positron emission tomography

Abstract

Keywords

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