TY - JOUR
T1 - Development and application of caged calcium
AU - Ellis-Davies, Graham C.R.
PY - 2003
Y1 - 2003
N2 - Several caged calciums have been synthesized since 1986, and three are commercially available: DM-nitrophen, NP-EGTA, and nitr-5. Each of these caged compounds has uniquely useful properties, making the choice of which cage to use dependent on the specific experiment (i.e., the cell type and divalent cation requirements of the experiments within purview). Significantly, methods have been developed for all three cages that permit their quantitative use inside many cell types, including those with some of the most demanding of requirements for experiments with caged calcium, namely, in relating presynaptic [Ca] to post-synaptic function. The success of such experiments using DM-nitrophen and NP-EGTA suggests that caged calcium is now a mature tool for cellular physiology and neurobiology.
AB - Several caged calciums have been synthesized since 1986, and three are commercially available: DM-nitrophen, NP-EGTA, and nitr-5. Each of these caged compounds has uniquely useful properties, making the choice of which cage to use dependent on the specific experiment (i.e., the cell type and divalent cation requirements of the experiments within purview). Significantly, methods have been developed for all three cages that permit their quantitative use inside many cell types, including those with some of the most demanding of requirements for experiments with caged calcium, namely, in relating presynaptic [Ca] to post-synaptic function. The success of such experiments using DM-nitrophen and NP-EGTA suggests that caged calcium is now a mature tool for cellular physiology and neurobiology.
UR - http://www.scopus.com/inward/record.url?scp=0037274759&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(03)60112-6
DO - 10.1016/S0076-6879(03)60112-6
M3 - Article
C2 - 12622152
AN - SCOPUS:0037274759
SN - 0076-6879
VL - 360
SP - 226
EP - 238
JO - Methods in Enzymology
JF - Methods in Enzymology
ER -