Determination of the rate of renaturation of modified DNA by fluorescence depolarization

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Abstract

Fluorescence depolarization was used to measure the rate of renaturation of T2 DNA, which had been modified by chloroacetaldehyde. Rates were measured on DNA samples with 5–15% of the base pairs modified and were found to agree with rates determined by DNA absorbance kinetics at 260 nm. The renaturation rate of a modified T2 DNA was unchanged in the presence of a ninefold abundance of unlabeled calf thymus DNA.

Original languageEnglish
Pages (from-to)2545-2551
Number of pages7
JournalBiopolymers
Volume13
Issue number12
DOIs
StatePublished - Dec 1974
Externally publishedYes

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