TY - JOUR
T1 - Determination of desmosines in elastin-related skin disorders by isocratic high-performance liquid chromatography
AU - Schwartz, Elaine
AU - Cruickshank, Frederick A.
AU - Lebwohl, Mark
N1 - Funding Information:
researob was supported by NIH Grant AR36101.
PY - 1990/2
Y1 - 1990/2
N2 - Abnormalities in the amount of skin elastin occur in several cutaneous disorders. The number of elastic fibers is increased in elastotic disorders such as pseudoxanthoma elasticum (PXE) and cutis rhomboidalis nuchae (actinic elastosis, AE) and is decreased in elastolytic disorders such as cutis laxa (CL). We describe a procedure to quantify desmosines and elastin in small amounts of skin using high-performance liquid chromatography (HPLC). Biopsies were obtained from normal, nonsolar exposed skin and from the lesional skin of patients with PXE, cutis rhomboidalis nuchae, and CL. Specimens were subjected to hot alkali treatment and the desmosines were released by acid hydrolysis and quantified by HPLC. The mean value for normal skin was 252 ± 28 ng desmosines per milligram wet weight (SD, n = 5). The disorders of elastosis (PXE and AE) demonstrated a two-to fivefold increased content of desmosines. In contrast, the elastolytic disorder (CL) had only 20% of the normal content of esmosines. Furthermore, PXE and normal skin elastins had the same amount of desmosines per milligram purified elastin. This method could be used to evaluate the extent of elastosis or elastolysis in a particular lesion.
AB - Abnormalities in the amount of skin elastin occur in several cutaneous disorders. The number of elastic fibers is increased in elastotic disorders such as pseudoxanthoma elasticum (PXE) and cutis rhomboidalis nuchae (actinic elastosis, AE) and is decreased in elastolytic disorders such as cutis laxa (CL). We describe a procedure to quantify desmosines and elastin in small amounts of skin using high-performance liquid chromatography (HPLC). Biopsies were obtained from normal, nonsolar exposed skin and from the lesional skin of patients with PXE, cutis rhomboidalis nuchae, and CL. Specimens were subjected to hot alkali treatment and the desmosines were released by acid hydrolysis and quantified by HPLC. The mean value for normal skin was 252 ± 28 ng desmosines per milligram wet weight (SD, n = 5). The disorders of elastosis (PXE and AE) demonstrated a two-to fivefold increased content of desmosines. In contrast, the elastolytic disorder (CL) had only 20% of the normal content of esmosines. Furthermore, PXE and normal skin elastins had the same amount of desmosines per milligram purified elastin. This method could be used to evaluate the extent of elastosis or elastolysis in a particular lesion.
UR - https://www.scopus.com/pages/publications/0025234436
U2 - 10.1016/0014-4800(90)90059-M
DO - 10.1016/0014-4800(90)90059-M
M3 - Article
C2 - 2307214
AN - SCOPUS:0025234436
SN - 0014-4800
VL - 52
SP - 63
EP - 68
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 1
ER -