TY - JOUR
T1 - Detection of the SYT-SSX chimeric RNA of synovial sarcoma in paraffin- embedded tissue and its application in problematic cases
AU - Argani, Pedram
AU - Zakowski, Maureen F.
AU - Klimstra, David S.
AU - Rosai, Juan
AU - Ladanyi, Marc
PY - 1998/1
Y1 - 1998/1
N2 - We report the development of a reverse-transcriptase polymerase chain reaction assay that detects (in paraffin-embedded, formalin-fixed tissue) the SYT-SSX chimeric RNA transcript resulting from the t(X;18) of synovial sarcoma. The primers chosen detect both of the SSX1 and SSX2 partners, and the target sequence is small enough (87 base pairs) to be reliably detected in archival and variably processed consultation material. To demonstrate its usefulness, we applied it to 14 problematic cases, including spindle cell tumors of the thoracic region, of the neck, and of subcutaneous tissue. For instance, we show that, depending on the location, synovial sarcoma can mimic malignant solitary fibrous rumor, the spindle epithelial tumor with thymus-like differentiation, or skin adnexal tumors. Molecular detection of the SYT-SSX chimeric RNA should allow the reclassification of difficult cases in which the morphologic features overlap different entities or in which tumor nosology is still evolving.
AB - We report the development of a reverse-transcriptase polymerase chain reaction assay that detects (in paraffin-embedded, formalin-fixed tissue) the SYT-SSX chimeric RNA transcript resulting from the t(X;18) of synovial sarcoma. The primers chosen detect both of the SSX1 and SSX2 partners, and the target sequence is small enough (87 base pairs) to be reliably detected in archival and variably processed consultation material. To demonstrate its usefulness, we applied it to 14 problematic cases, including spindle cell tumors of the thoracic region, of the neck, and of subcutaneous tissue. For instance, we show that, depending on the location, synovial sarcoma can mimic malignant solitary fibrous rumor, the spindle epithelial tumor with thymus-like differentiation, or skin adnexal tumors. Molecular detection of the SYT-SSX chimeric RNA should allow the reclassification of difficult cases in which the morphologic features overlap different entities or in which tumor nosology is still evolving.
KW - Chromosomal translocation
KW - Polymerase chain reaction
KW - Solitary fibrous tumor
KW - Synovial sarcoma
UR - https://www.scopus.com/pages/publications/0031966030
M3 - Article
C2 - 9556426
AN - SCOPUS:0031966030
SN - 0893-3952
VL - 11
SP - 65
EP - 71
JO - Modern Pathology
JF - Modern Pathology
IS - 1
ER -