@article{f3951872d2b548c48e330e8890a9a8c3,
title = "Detection of single base substitutions in influenza virus RNA molecules by denaturing gradient gel electrophoresis of RNA-RNA or DNA-RNA heteroduplexes",
abstract = "Single point mutations in the NS gene of influenza virus were detected by electrophoresis of double-stranded RNA heteroduplexes in denaturing gradient gels. The heteroduplex RNAs were made by hybridization of virion RNA with SP6-derived RNA probes of varying length. Mutations located at different positions along the NS gene (890 nucleotides long) were all detected in a predictable fashion. The method of heteroduplex analysis was also successfully used in detecting single point mismatches in DNA-RNA hybrids.",
author = "Smith, {Frances I.} and Parvin, {Jeffrey D.} and Peter Palese",
note = "Funding Information: We thank Dr. L. Lerman and Dr. K. Silverstein (Genetics Institute, Boston, Mass.) for helpful discussion and for graciously supplying us with the melting map program, and Dr. Seth Pinsky (Rockefeller University, N. Y.) for sharing his computer expertise. We also thank Mr. Robert Dachert of Ther-matron/Thermex, Inc., Bayshore, N. Y., for stimulating discussions and technical advice. F.I.S. is a Special Fellow of the Leukemia Society of America. J.D.P. is supported by Medical Scientist Training Grant GM07280 from the National Institutes of General Medical Sciences. This work was supported by National Institutes of Health Grants AI-11823 and AI-18998.",
year = "1986",
month = apr,
day = "15",
doi = "10.1016/0042-6822(86)90265-5",
language = "English",
volume = "150",
pages = "55--64",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",
}