Detection of single base substitutions in influenza virus RNA molecules by denaturing gradient gel electrophoresis of RNA-RNA or DNA-RNA heteroduplexes

Frances I. Smith, Jeffrey D. Parvin, Peter Palese

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Single point mutations in the NS gene of influenza virus were detected by electrophoresis of double-stranded RNA heteroduplexes in denaturing gradient gels. The heteroduplex RNAs were made by hybridization of virion RNA with SP6-derived RNA probes of varying length. Mutations located at different positions along the NS gene (890 nucleotides long) were all detected in a predictable fashion. The method of heteroduplex analysis was also successfully used in detecting single point mismatches in DNA-RNA hybrids.

Original languageEnglish
Pages (from-to)55-64
Number of pages10
JournalVirology
Volume150
Issue number1
DOIs
StatePublished - 15 Apr 1986
Externally publishedYes

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