TY - JOUR
T1 - Detection of respiratory viruses and subtype identification of influenza A viruses by greenechipresp oligonucleotide microarray
AU - Quan, Phenix Lan
AU - Palacios, Gustavo
AU - Jabado, Omar J.
AU - Conlan, Sean
AU - Hirschberg, David L.
AU - Pozo, Francisco
AU - Jack, Philippa J.M.
AU - Cisterna, Daniel
AU - Renwick, Neil
AU - Hui, Jeffrey
AU - Drysdale, Andrew
AU - Amos-Ritchie, Rachel
AU - Baumeister, Elsa
AU - Savy, Vilma
AU - Lager, Kelly M.
AU - Richt, Jürgen A.
AU - Boyle, David B.
AU - García-Sastre, Adolfo
AU - Casas, Inmaculada
AU - Perez-Breña, Pilar
AU - Briese, Thomas
AU - Lipkin, W. Ian
PY - 2007/8
Y1 - 2007/8
N2 - Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus.
AB - Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus.
UR - http://www.scopus.com/inward/record.url?scp=34548084879&partnerID=8YFLogxK
U2 - 10.1128/JCM.00737-07
DO - 10.1128/JCM.00737-07
M3 - Article
C2 - 17553978
AN - SCOPUS:34548084879
SN - 0095-1137
VL - 45
SP - 2359
EP - 2364
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 8
ER -