Detection of hepatitis C virus RNA sequences in cholangiocarcinomas in Chinese and American patients

Huiying Lu; Michele Q. Ye; Swan N. Thung; Srikanta Dash; Michael A. Gerber

Detection of hepatitis C virus RNA sequences in cholangiocarcinomas in Chinese and American patients

Huiying Lu, Michele Q. Ye, Swan N. Thung, Srikanta Dash, Michael A. Gerber

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Objective: To investigate the role of hepatitis C virus (HCV) in the malignant transformation of bile duct cells. Tissues from 6 Chinese patients and 6 American patients with cholangiocarcinoma were studied. Methods: RNA was extracted from the selected tumor areas of formalin-fixed, paraffin embedded sections, followed by reverse transcription double polymerase chain reaction (RT-PCR) and Southern blotting. Results: Positive and negative strand HCV RNA sequences were detected in seven out of twelve patients with cholangiocarcinoma. A high positive rate was found in Chinese patients (83%) as compared to US patients (33%). Conclusion: Our finding suggests HCV may play a role in the malignant transformation of bile duct cells.

Original language	English
Pages (from-to)	1138-1141
Number of pages	4
Journal	Chinese Medical Journal
Volume	113
Issue number	12
State	Published - Dec 2000

Keywords

Bile ducts
Cholangiocarcinoma
Hepatitis C virus
Reverse transcription polymerase chain reaction
Southern blotting

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title = "Detection of hepatitis C virus RNA sequences in cholangiocarcinomas in Chinese and American patients",

abstract = "Objective: To investigate the role of hepatitis C virus (HCV) in the malignant transformation of bile duct cells. Tissues from 6 Chinese patients and 6 American patients with cholangiocarcinoma were studied. Methods: RNA was extracted from the selected tumor areas of formalin-fixed, paraffin embedded sections, followed by reverse transcription double polymerase chain reaction (RT-PCR) and Southern blotting. Results: Positive and negative strand HCV RNA sequences were detected in seven out of twelve patients with cholangiocarcinoma. A high positive rate was found in Chinese patients (83%) as compared to US patients (33%). Conclusion: Our finding suggests HCV may play a role in the malignant transformation of bile duct cells.",

keywords = "Bile ducts, Cholangiocarcinoma, Hepatitis C virus, Reverse transcription polymerase chain reaction, Southern blotting",

author = "Huiying Lu and Ye, {Michele Q.} and Thung, {Swan N.} and Srikanta Dash and Gerber, {Michael A.}",

year = "2000",

month = dec,

language = "English",

volume = "113",

pages = "1138--1141",

journal = "Chinese Medical Journal",

issn = "0366-6999",

publisher = "Lippincott Williams and Wilkins",

number = "12",

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TY - JOUR

T1 - Detection of hepatitis C virus RNA sequences in cholangiocarcinomas in Chinese and American patients

AU - Lu, Huiying

AU - Ye, Michele Q.

AU - Thung, Swan N.

AU - Dash, Srikanta

AU - Gerber, Michael A.

PY - 2000/12

Y1 - 2000/12

N2 - Objective: To investigate the role of hepatitis C virus (HCV) in the malignant transformation of bile duct cells. Tissues from 6 Chinese patients and 6 American patients with cholangiocarcinoma were studied. Methods: RNA was extracted from the selected tumor areas of formalin-fixed, paraffin embedded sections, followed by reverse transcription double polymerase chain reaction (RT-PCR) and Southern blotting. Results: Positive and negative strand HCV RNA sequences were detected in seven out of twelve patients with cholangiocarcinoma. A high positive rate was found in Chinese patients (83%) as compared to US patients (33%). Conclusion: Our finding suggests HCV may play a role in the malignant transformation of bile duct cells.

AB - Objective: To investigate the role of hepatitis C virus (HCV) in the malignant transformation of bile duct cells. Tissues from 6 Chinese patients and 6 American patients with cholangiocarcinoma were studied. Methods: RNA was extracted from the selected tumor areas of formalin-fixed, paraffin embedded sections, followed by reverse transcription double polymerase chain reaction (RT-PCR) and Southern blotting. Results: Positive and negative strand HCV RNA sequences were detected in seven out of twelve patients with cholangiocarcinoma. A high positive rate was found in Chinese patients (83%) as compared to US patients (33%). Conclusion: Our finding suggests HCV may play a role in the malignant transformation of bile duct cells.

KW - Bile ducts

KW - Cholangiocarcinoma

KW - Hepatitis C virus

KW - Reverse transcription polymerase chain reaction

KW - Southern blotting

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M3 - Article

C2 - 11776153

AN - SCOPUS:0034585440

SN - 0366-6999

VL - 113

SP - 1138

EP - 1141

JO - Chinese Medical Journal

JF - Chinese Medical Journal

IS - 12

ER -

Detection of hepatitis C virus RNA sequences in cholangiocarcinomas in Chinese and American patients

Abstract

Keywords

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