Detection of glycosaminoglycans as a copper(II) complex in high performance liquid chromatography

Toshihiko Toida; Makiko Shima; Saori Azumaya; Tomohiro Maruyama; Hidenao Toyoda; Toshio Imanari; Robert J. Linhardt

doi:10.1016/S0021-9673(97)00658-4

Detection of glycosaminoglycans as a copper(II) complex in high performance liquid chromatography

Toshihiko Toida, Makiko Shima, Saori Azumaya, Tomohiro Maruyama, Hidenao Toyoda, Toshio Imanari, Robert J. Linhardt

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10^-7 g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N- acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.

Original language	English
Pages (from-to)	266-270
Number of pages	5
Journal	Journal of Chromatography A
Volume	787
Issue number	1-2
DOIs	https://doi.org/10.1016/S0021-9673(97)00658-4
State	Published - 7 Nov 1997
Externally published	Yes

Keywords

Copper complexes
Derivatization
Detection
Glycans
Glycosaminoglycans
LC
Metal complexes

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10.1016/S0021-9673(97)00658-4

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title = "Detection of glycosaminoglycans as a copper(II) complex in high performance liquid chromatography",

abstract = "Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10-7 g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N- acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.",

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T1 - Detection of glycosaminoglycans as a copper(II) complex in high performance liquid chromatography

AU - Toida, Toshihiko

AU - Shima, Makiko

AU - Azumaya, Saori

AU - Maruyama, Tomohiro

AU - Toyoda, Hidenao

AU - Imanari, Toshio

AU - Linhardt, Robert J.

PY - 1997/11/7

Y1 - 1997/11/7

N2 - Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10-7 g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N- acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.

AB - Glycosaminoglycans including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate and hyaluronan were analyzed by high-performance gel filtration chromatography. Detection was achieved at 240 nm based on the formation of copper(II) complex in copper sulfate solution at low pH. Detection of the copper(II)-heparin complex is sensitive, permitting the analysis of as little as 10-7 g. This method was also successfully applied to the analysis of the chemically derivatized glycosaminoglycans (de-N- acetylated and/or oversulfated chondroitin sulfate) that cannot be depolymerized by enzymes such as heparin and chondroitin lyases.

KW - Copper complexes

KW - Derivatization

KW - Detection

KW - Glycans

KW - Glycosaminoglycans

KW - LC

KW - Metal complexes

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Detection of glycosaminoglycans as a copper(II) complex in high performance liquid chromatography

Abstract

Keywords

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