TY - JOUR
T1 - Detection of experimental myocarditis by monoclonal antimyosin antibody, Fab fragment
AU - Rezkalla, Shereif
AU - Kloner, Robert A.
AU - Khaw, Ban An
AU - Haber, Edgar
AU - Fallon, John T.
AU - Smith, Frederick E.
AU - Khatib, Riad
PY - 1989/2
Y1 - 1989/2
N2 - The purpose of this study was to determine whether monoclonal antimyosin Fab (antigen binding fragment) was capable of labeling hearts with experimental coxsackievirus myocarditis, and to determine whether Fab could be used for detecting myocardial damage in either early or chronic phases of the disease. Sixty-five, 3-week-old cesarean-derived 1 (CD 1) mice were divided into two groups: group I (noninfected animals) and group II (infected with coxsackievirus B3). Mice from each group were killed on days 7, 17, 30, or 90 of infection. Forty-eight hours before killing, mice were injected with monoclonal I125 antimyosin, Fab (25 μCl/injection) and radioactivity was counted in the heart. Selected heart sections were also examined by autoradiography. Heart radioactivity, count/m/mg (m ± SEM) on days 7, 17, 30, and 90 of infection was 10.8 ± 1.7, 21.3 ± 1.1, 11.2 ± 3.4, and 12.4 ± 1.5 for group I, versus 36.7 ± 8.0 (p < 0.01), 50.0 ± 4.5 (p < 0.001), 33.4 ± 16.1 (p = NS), and 40.6 ± 8.5 (p < 0.01) for group II, respectively. Autoradiography revealed focal uptake within areas of necrotic myocardium. We conclude that I125 Fab may be useful in detecting myocardial damage in the experimental model of murine myocarditis up to day 90 of infection.
AB - The purpose of this study was to determine whether monoclonal antimyosin Fab (antigen binding fragment) was capable of labeling hearts with experimental coxsackievirus myocarditis, and to determine whether Fab could be used for detecting myocardial damage in either early or chronic phases of the disease. Sixty-five, 3-week-old cesarean-derived 1 (CD 1) mice were divided into two groups: group I (noninfected animals) and group II (infected with coxsackievirus B3). Mice from each group were killed on days 7, 17, 30, or 90 of infection. Forty-eight hours before killing, mice were injected with monoclonal I125 antimyosin, Fab (25 μCl/injection) and radioactivity was counted in the heart. Selected heart sections were also examined by autoradiography. Heart radioactivity, count/m/mg (m ± SEM) on days 7, 17, 30, and 90 of infection was 10.8 ± 1.7, 21.3 ± 1.1, 11.2 ± 3.4, and 12.4 ± 1.5 for group I, versus 36.7 ± 8.0 (p < 0.01), 50.0 ± 4.5 (p < 0.001), 33.4 ± 16.1 (p = NS), and 40.6 ± 8.5 (p < 0.01) for group II, respectively. Autoradiography revealed focal uptake within areas of necrotic myocardium. We conclude that I125 Fab may be useful in detecting myocardial damage in the experimental model of murine myocarditis up to day 90 of infection.
UR - https://www.scopus.com/pages/publications/0024533654
U2 - 10.1016/0002-8703(89)90785-0
DO - 10.1016/0002-8703(89)90785-0
M3 - Article
C2 - 2916411
AN - SCOPUS:0024533654
SN - 0002-8703
VL - 117
SP - 391
EP - 395
JO - American Heart Journal
JF - American Heart Journal
IS - 2
ER -