Detection of experimental myocarditis by monoclonal antimyosin antibody, Fab fragment

Shereif Rezkalla, Robert A. Kloner, Ban An Khaw, Edgar Haber, John T. Fallon, Frederick E. Smith, Riad Khatib

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The purpose of this study was to determine whether monoclonal antimyosin Fab (antigen binding fragment) was capable of labeling hearts with experimental coxsackievirus myocarditis, and to determine whether Fab could be used for detecting myocardial damage in either early or chronic phases of the disease. Sixty-five, 3-week-old cesarean-derived 1 (CD 1) mice were divided into two groups: group I (noninfected animals) and group II (infected with coxsackievirus B3). Mice from each group were killed on days 7, 17, 30, or 90 of infection. Forty-eight hours before killing, mice were injected with monoclonal I125 antimyosin, Fab (25 μCl/injection) and radioactivity was counted in the heart. Selected heart sections were also examined by autoradiography. Heart radioactivity, count/m/mg (m ± SEM) on days 7, 17, 30, and 90 of infection was 10.8 ± 1.7, 21.3 ± 1.1, 11.2 ± 3.4, and 12.4 ± 1.5 for group I, versus 36.7 ± 8.0 (p < 0.01), 50.0 ± 4.5 (p < 0.001), 33.4 ± 16.1 (p = NS), and 40.6 ± 8.5 (p < 0.01) for group II, respectively. Autoradiography revealed focal uptake within areas of necrotic myocardium. We conclude that I125 Fab may be useful in detecting myocardial damage in the experimental model of murine myocarditis up to day 90 of infection.

Original languageEnglish
Pages (from-to)391-395
Number of pages5
JournalAmerican Heart Journal
Volume117
Issue number2
DOIs
StatePublished - Feb 1989
Externally publishedYes

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