TY - JOUR
T1 - Detection of Epstein-Barr virus (EBV) DNA sequences using in situ hybridization
AU - Moar, M. H.
AU - Klein, G.
N1 - Funding Information:
This work was supported by Contract Grant Number NO1 CP 33316 within the virus Cancer Programme of the National Cancer Institute, the Swedish Cancer Society, and the European Molecular Biology Organization (EMBO) of which M.H.M. was a Fellowship recipient. We thank Ors. T. Lindahl and S. Koliais for reading the manusscript.
PY - 1978/6/22
Y1 - 1978/6/22
N2 - In situ hybridization was used to detect Epstein-Barr virus (EBV) DNA sequences under conditions where the virus DNA is replicating spontaneously and where it is induced to do so following superinfection. The in situ reaction itself is influenced by several parameters, analogous to conventional nucleic acid hybridization, consideration of which should help to optimize the designing of in situ hybridization reactions in general. Both EBV complementary RNA (cRNA) and EBV DNA synthesized in vitro can efficiently detect the virus DNA sequences in situ. The findings presented here can therefore be utilized in both the study of EBV-cell interactions and, more generally, in studies using in situ hybridization as a general approach.
AB - In situ hybridization was used to detect Epstein-Barr virus (EBV) DNA sequences under conditions where the virus DNA is replicating spontaneously and where it is induced to do so following superinfection. The in situ reaction itself is influenced by several parameters, analogous to conventional nucleic acid hybridization, consideration of which should help to optimize the designing of in situ hybridization reactions in general. Both EBV complementary RNA (cRNA) and EBV DNA synthesized in vitro can efficiently detect the virus DNA sequences in situ. The findings presented here can therefore be utilized in both the study of EBV-cell interactions and, more generally, in studies using in situ hybridization as a general approach.
UR - https://www.scopus.com/pages/publications/0017899919
U2 - 10.1016/0005-2787(78)90061-8
DO - 10.1016/0005-2787(78)90061-8
M3 - Article
C2 - 208612
AN - SCOPUS:0017899919
SN - 0005-2787
VL - 519
SP - 49
EP - 64
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 1
ER -