Detection and characterization of EBV antigens by micro-ELISA and chromatofocusing

Lars Sternås; Janos Luka; Bengt Kallin; Bent Faber Vestergaard; George Klein

doi:10.1016/0166-0934(82)90069-6

Detection and characterization of EBV antigens by micro-ELISA and chromatofocusing

Lars Sternås
, Janos Luka
, Bengt Kallin
, Bent Faber Vestergaard
, George Klein

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A micro-ELISA technique was developed for the detection of Epstein-Barr virus (EBV)-determined antigens. The enzyme-linked immunosorbent assay (ELISA) was applied with peroxidase-protein A to detect the antigens adsorbed to micro-ELISA plates. Human and rabbit antisera containing antibodies to known EBV components were used as reagents. The early antigen (EA) complex, associated with the viral cycle, was readily detected in extracts of n-butyrate- or n-butyrate + TPA-induced cells. The nuclear antigen, EBNA, could be unequivocally detected only after the partial purification of the antigen by DNA cellulose chromatography. EA (and VCA) could be separated by chromatofocusing of induced cell extracts into several fractions detected by the micro-ELISA technique. This indicates that the purification of individual antigens of the EA complex can be monitored by ELISA.

Original language	English
Pages (from-to)	229-240
Number of pages	12
Journal	Journal of Virological Methods
Volume	4
Issue number	4-5
DOIs	https://doi.org/10.1016/0166-0934(82)90069-6
State	Published - May 1982
Externally published	Yes

Keywords

EBV antigens
chromatofocusing
micro-ELISA

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10.1016/0166-0934(82)90069-6

Cite this

@article{7e65c92763ce4443b5df7beb111eaef9,

title = "Detection and characterization of EBV antigens by micro-ELISA and chromatofocusing",

abstract = "A micro-ELISA technique was developed for the detection of Epstein-Barr virus (EBV)-determined antigens. The enzyme-linked immunosorbent assay (ELISA) was applied with peroxidase-protein A to detect the antigens adsorbed to micro-ELISA plates. Human and rabbit antisera containing antibodies to known EBV components were used as reagents. The early antigen (EA) complex, associated with the viral cycle, was readily detected in extracts of n-butyrate- or n-butyrate + TPA-induced cells. The nuclear antigen, EBNA, could be unequivocally detected only after the partial purification of the antigen by DNA cellulose chromatography. EA (and VCA) could be separated by chromatofocusing of induced cell extracts into several fractions detected by the micro-ELISA technique. This indicates that the purification of individual antigens of the EA complex can be monitored by ELISA.",

keywords = "EBV antigens, chromatofocusing, micro-ELISA",

author = "Lars Stern{\aa}s and Janos Luka and Bengt Kallin and Vestergaard, \{Bent Faber\} and George Klein",

note = "Funding Information: This \textasciitilde{}vestigat\textasciitilde{}onw as supportedb y PHS grant No. I ROl \&A 28380-01 Al awarded by the National Cancer Institute, DHHS, the Swedish Cancer Society and King Gustaf V Jubilee Fund.",

year = "1982",

month = may,

doi = "10.1016/0166-0934(82)90069-6",

language = "English",

volume = "4",

pages = "229--240",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "4-5",

}

TY - JOUR

T1 - Detection and characterization of EBV antigens by micro-ELISA and chromatofocusing

AU - Sternås, Lars

AU - Luka, Janos

AU - Kallin, Bengt

AU - Vestergaard, Bent Faber

AU - Klein, George

N1 - Funding Information: This ~vestigat~onw as supportedb y PHS grant No. I ROl &A 28380-01 Al awarded by the National Cancer Institute, DHHS, the Swedish Cancer Society and King Gustaf V Jubilee Fund.

PY - 1982/5

Y1 - 1982/5

N2 - A micro-ELISA technique was developed for the detection of Epstein-Barr virus (EBV)-determined antigens. The enzyme-linked immunosorbent assay (ELISA) was applied with peroxidase-protein A to detect the antigens adsorbed to micro-ELISA plates. Human and rabbit antisera containing antibodies to known EBV components were used as reagents. The early antigen (EA) complex, associated with the viral cycle, was readily detected in extracts of n-butyrate- or n-butyrate + TPA-induced cells. The nuclear antigen, EBNA, could be unequivocally detected only after the partial purification of the antigen by DNA cellulose chromatography. EA (and VCA) could be separated by chromatofocusing of induced cell extracts into several fractions detected by the micro-ELISA technique. This indicates that the purification of individual antigens of the EA complex can be monitored by ELISA.

AB - A micro-ELISA technique was developed for the detection of Epstein-Barr virus (EBV)-determined antigens. The enzyme-linked immunosorbent assay (ELISA) was applied with peroxidase-protein A to detect the antigens adsorbed to micro-ELISA plates. Human and rabbit antisera containing antibodies to known EBV components were used as reagents. The early antigen (EA) complex, associated with the viral cycle, was readily detected in extracts of n-butyrate- or n-butyrate + TPA-induced cells. The nuclear antigen, EBNA, could be unequivocally detected only after the partial purification of the antigen by DNA cellulose chromatography. EA (and VCA) could be separated by chromatofocusing of induced cell extracts into several fractions detected by the micro-ELISA technique. This indicates that the purification of individual antigens of the EA complex can be monitored by ELISA.

KW - EBV antigens

KW - chromatofocusing

KW - micro-ELISA

UR - https://www.scopus.com/pages/publications/0019950243

U2 - 10.1016/0166-0934(82)90069-6

DO - 10.1016/0166-0934(82)90069-6

M3 - Article

C2 - 6286703

AN - SCOPUS:0019950243

SN - 0166-0934

VL - 4

SP - 229

EP - 240

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 4-5

ER -

Detection and characterization of EBV antigens by micro-ELISA and chromatofocusing

Abstract

Keywords

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