Detecting of non-histone proteins bounded to the regulatory elements of yeast rRNA genes by UV-induced links on intact nuclei

D. A. Papatsenko; I. V. Priporova; A. V. Kukarin; O. V. Preobrazhenskaya; V. L. Karpov; A. D. Mirzabekov

Detecting of non-histone proteins bounded to the regulatory elements of yeast rRNA genes by UV-induced links on intact nuclei

D. A. Papatsenko
, I. V. Priporova
, A. V. Kukarin
, O. V. Preobrazhenskaya
, V. L. Karpov
, A. D. Mirzabekov

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We have studied the arrangement of DNA-binding proteins along yeast ribosomal non-transcribed space by UV-induced DNA-protein crosslinking on intact nuclei. We show binding of proteins with apparent Mw 120 and 30 kDa in promoter region, 120 kDa in enhancer region, 40 kDa in ARS. These proteins were identified preliminary as REB1 (promoter; enhancer), TFID (promoter), MCM3 (ARS). Good agreement between information supplied by this technics and literature data prove usefulness of our approach and make possibility for its wide appliance.

Original language	English
Pages (from-to)	1376-1382
Number of pages	7
Journal	Molekulyarnaya Biologiya
Volume	28
Issue number	6
State	Published - 1994
Externally published	Yes

Cite this

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title = "Detecting of non-histone proteins bounded to the regulatory elements of yeast rRNA genes by UV-induced links on intact nuclei",

abstract = "We have studied the arrangement of DNA-binding proteins along yeast ribosomal non-transcribed space by UV-induced DNA-protein crosslinking on intact nuclei. We show binding of proteins with apparent Mw 120 and 30 kDa in promoter region, 120 kDa in enhancer region, 40 kDa in ARS. These proteins were identified preliminary as REB1 (promoter; enhancer), TFID (promoter), MCM3 (ARS). Good agreement between information supplied by this technics and literature data prove usefulness of our approach and make possibility for its wide appliance.",

author = "Papatsenko, \{D. A.\} and Priporova, \{I. V.\} and Kukarin, \{A. V.\} and Preobrazhenskaya, \{O. V.\} and Karpov, \{V. L.\} and Mirzabekov, \{A. D.\}",

year = "1994",

language = "English",

volume = "28",

pages = "1376--1382",

journal = "Molekulyarnaya Biologiya",

issn = "0026-8984",

publisher = "Russian Academy of Sciences",

number = "6",

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TY - JOUR

T1 - Detecting of non-histone proteins bounded to the regulatory elements of yeast rRNA genes by UV-induced links on intact nuclei

AU - Papatsenko, D. A.

AU - Priporova, I. V.

AU - Kukarin, A. V.

AU - Preobrazhenskaya, O. V.

AU - Karpov, V. L.

AU - Mirzabekov, A. D.

PY - 1994

Y1 - 1994

N2 - We have studied the arrangement of DNA-binding proteins along yeast ribosomal non-transcribed space by UV-induced DNA-protein crosslinking on intact nuclei. We show binding of proteins with apparent Mw 120 and 30 kDa in promoter region, 120 kDa in enhancer region, 40 kDa in ARS. These proteins were identified preliminary as REB1 (promoter; enhancer), TFID (promoter), MCM3 (ARS). Good agreement between information supplied by this technics and literature data prove usefulness of our approach and make possibility for its wide appliance.

AB - We have studied the arrangement of DNA-binding proteins along yeast ribosomal non-transcribed space by UV-induced DNA-protein crosslinking on intact nuclei. We show binding of proteins with apparent Mw 120 and 30 kDa in promoter region, 120 kDa in enhancer region, 40 kDa in ARS. These proteins were identified preliminary as REB1 (promoter; enhancer), TFID (promoter), MCM3 (ARS). Good agreement between information supplied by this technics and literature data prove usefulness of our approach and make possibility for its wide appliance.

UR - https://www.scopus.com/pages/publications/0028619939

M3 - Article

C2 - 7885337

AN - SCOPUS:0028619939

SN - 0026-8984

VL - 28

SP - 1376

EP - 1382

JO - Molekulyarnaya Biologiya

JF - Molekulyarnaya Biologiya

IS - 6

ER -

Detecting of non-histone proteins bounded to the regulatory elements of yeast rRNA genes by UV-induced links on intact nuclei

Abstract

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