TY - CHAP
T1 - Detecting and Modulating ER Stress to Improve Generation of Induced Pluripotent Stem Cells
AU - Fuentes-Iglesias, Alejandro
AU - Ameneiro, Cristina
AU - Guallar, Diana
AU - Fidalgo, Miguel
N1 - Publisher Copyright:
© 2021, Springer Science+Business Media, LLC.
PY - 2022
Y1 - 2022
N2 - The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) has proven to be a powerful system creating new opportunities to interrogate molecular mechanisms controlling cell fate determination. Under standard conditions, the generation of iPSCs upon overexpression of OCT4, SOX2, KLF4, and c-MYC (OSKM) is generally slow and inefficient due to the presence of barriers that confer resistance to cell fate changes. Hyperactivated endoplasmic reticulum (ER) stress has emerged as a major reprogramming barrier that impedes the initial mesenchymal-to-epithelial transition (MET) step to form iPSCs from mesenchymal somatic cells. Here, we describe several systems to detect ER stress in the context of OSKM reprogramming and chemical interventions to modulate this process for improving iPSC formation.
AB - The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) has proven to be a powerful system creating new opportunities to interrogate molecular mechanisms controlling cell fate determination. Under standard conditions, the generation of iPSCs upon overexpression of OCT4, SOX2, KLF4, and c-MYC (OSKM) is generally slow and inefficient due to the presence of barriers that confer resistance to cell fate changes. Hyperactivated endoplasmic reticulum (ER) stress has emerged as a major reprogramming barrier that impedes the initial mesenchymal-to-epithelial transition (MET) step to form iPSCs from mesenchymal somatic cells. Here, we describe several systems to detect ER stress in the context of OSKM reprogramming and chemical interventions to modulate this process for improving iPSC formation.
KW - Cell fate change
KW - Endoplasmic reticulum stress
KW - Induced pluripotent stem cell
KW - Mesenchymal-to-epithelial transition
KW - Pluripotency
KW - Unfolded protein response
UR - http://www.scopus.com/inward/record.url?scp=85132455858&partnerID=8YFLogxK
U2 - 10.1007/7651_2021_354
DO - 10.1007/7651_2021_354
M3 - Chapter
C2 - 33689163
AN - SCOPUS:85132455858
T3 - Methods in Molecular Biology
SP - 743
EP - 754
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -