Design and Production of Newcastle Disease Virus for Intratumoral Immunomodulation

Gayathri Vijayakumar, Dmitriy Zamarin

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

11 Scopus citations


Newcastle disease virus (NDV) is an avian paramyxovirus that has been extensively studied as an oncolytic agent, in addition to being an economically important pathogen in the poultry industry. The establishment of a reverse genetics system for this virus has enabled the development of genetically modified recombinant NDV viruses with improved oncolytic and immunotherapeutic properties. In this chapter, we describe the materials and methods involved in the in vitro cloning and rescue of NDV expressing murine 4-1BBL as well as the in vivo evaluation of NDV expressing 4-1BBL in a B16-F10 murine melanoma model.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages22
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Immunotherapy
  • Newcastle disease virus
  • Oncolytic vector
  • Reverse genetics
  • Virus rescue


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