TY - JOUR
T1 - Depressed oxidative metabolism in skeletal muscle after chronic alcohol consumption
AU - Shaw, Spencer
AU - Leo, Maria Anna
AU - Jayatilleke, Elizabeth
AU - Lieber, Charles S.
N1 - Funding Information:
We gratefully acknowledge the expert assistance which the LEMSIP staff provided for the baboon studies and Steven A. Mortillo for the technical help with the electron microscopy. The work in this study was supported by the Veterans Administration and USPHS Grants AA-03508 and AA-00698.
PY - 1982/10
Y1 - 1982/10
N2 - Morphological and biochemical studies were performed on skeletal muscle obtained from rats and baboons fed alcohol chronically along with an adequate diet. Muscle mitochondria from both species demonstrated marked alterations: enlargement and distortion of shape, loss of cristae, and vacuolization of the matrix. Following an overnight fast, when alcohol was no longer measurable in the blood, respiration in muscle slices obtained from the rats was depressed 25-40% (P < 0.01-0.05) both in the presence and absence of added substrate. Isolated mitochondria from rats and baboons demonstrated depressions in oxygen consumption ranging from 45 to 60%. These changes were present with various substrates: glutamate (10 mm), glycerol-1-phosphate (10 mm), and malonate with alpha-ketoglutarate (5 mm + 5mm). Changes were similar in States 3 and 4 of respiration. ADP:O ratios and respiratory control ratios were not significantly different in alcohol-fed and control animals. Thus, chronic alcohol consumption results in morphological changes in muscle mitochondria associated with a marked depression in oxidative metabolism.
AB - Morphological and biochemical studies were performed on skeletal muscle obtained from rats and baboons fed alcohol chronically along with an adequate diet. Muscle mitochondria from both species demonstrated marked alterations: enlargement and distortion of shape, loss of cristae, and vacuolization of the matrix. Following an overnight fast, when alcohol was no longer measurable in the blood, respiration in muscle slices obtained from the rats was depressed 25-40% (P < 0.01-0.05) both in the presence and absence of added substrate. Isolated mitochondria from rats and baboons demonstrated depressions in oxygen consumption ranging from 45 to 60%. These changes were present with various substrates: glutamate (10 mm), glycerol-1-phosphate (10 mm), and malonate with alpha-ketoglutarate (5 mm + 5mm). Changes were similar in States 3 and 4 of respiration. ADP:O ratios and respiratory control ratios were not significantly different in alcohol-fed and control animals. Thus, chronic alcohol consumption results in morphological changes in muscle mitochondria associated with a marked depression in oxidative metabolism.
UR - http://www.scopus.com/inward/record.url?scp=0019922052&partnerID=8YFLogxK
U2 - 10.1016/0006-2944(82)90072-2
DO - 10.1016/0006-2944(82)90072-2
M3 - Article
C2 - 7181871
AN - SCOPUS:0019922052
SN - 0006-2944
VL - 28
SP - 210
EP - 223
JO - Biochemical Medicine
JF - Biochemical Medicine
IS - 2
ER -