Denaturation and Renaturation of Penicillium chrysogenum Mycophage Double-Stranded Ribonucleic Acid in Tetraalkylammonium Salt Solutions

James G. Wetmur; William T. Ruyechan; Richard J. Douthart

doi:10.1021/bi00514a002

Denaturation and Renaturation of Penicillium chrysogenum Mycophage Double-Stranded Ribonucleic Acid in Tetraalkylammonium Salt Solutions

James G. Wetmur, William T. Ruyechan, Richard J. Douthart

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The base composition dependence of doublestranded ribonucleic acid (RNA) melting was studied by observing the structure and widths of melting transitions for Penicillium chrysogenum mycophage RNA as well as differences in melting temperatures of two RNAs of different base composition. Double-stranded RNA melting is independent of base compositions in 3.5 M Et4NCl and 4.6 M Me4NCl, where the melting temperatures are 25 and 92 °C, respectively. Double-stranded RNA renaturation rate constants are reported in Et4NCl solutions. Th e nucleation rate constant is about 10 times lower than that for double-stranded deoxyribonucleic acid. Analyses of renaturation kinetics results lead to the conclusion that each of the three similar but separable RNA segments of Penicillium chrysogenum mycophage is unique.

Original language	English
Pages (from-to)	2999-3002
Number of pages	4
Journal	Biochemistry
Volume	20
Issue number	11
DOIs	https://doi.org/10.1021/bi00514a002
State	Published - May 1981
Externally published	Yes

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title = "Denaturation and Renaturation of Penicillium chrysogenum Mycophage Double-Stranded Ribonucleic Acid in Tetraalkylammonium Salt Solutions",

abstract = "The base composition dependence of doublestranded ribonucleic acid (RNA) melting was studied by observing the structure and widths of melting transitions for Penicillium chrysogenum mycophage RNA as well as differences in melting temperatures of two RNAs of different base composition. Double-stranded RNA melting is independent of base compositions in 3.5 M Et4NCl and 4.6 M Me4NCl, where the melting temperatures are 25 and 92 °C, respectively. Double-stranded RNA renaturation rate constants are reported in Et4NCl solutions. Th e nucleation rate constant is about 10 times lower than that for double-stranded deoxyribonucleic acid. Analyses of renaturation kinetics results lead to the conclusion that each of the three similar but separable RNA segments of Penicillium chrysogenum mycophage is unique.",

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T1 - Denaturation and Renaturation of Penicillium chrysogenum Mycophage Double-Stranded Ribonucleic Acid in Tetraalkylammonium Salt Solutions

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AU - Ruyechan, William T.

AU - Douthart, Richard J.

PY - 1981/5

Y1 - 1981/5

N2 - The base composition dependence of doublestranded ribonucleic acid (RNA) melting was studied by observing the structure and widths of melting transitions for Penicillium chrysogenum mycophage RNA as well as differences in melting temperatures of two RNAs of different base composition. Double-stranded RNA melting is independent of base compositions in 3.5 M Et4NCl and 4.6 M Me4NCl, where the melting temperatures are 25 and 92 °C, respectively. Double-stranded RNA renaturation rate constants are reported in Et4NCl solutions. Th e nucleation rate constant is about 10 times lower than that for double-stranded deoxyribonucleic acid. Analyses of renaturation kinetics results lead to the conclusion that each of the three similar but separable RNA segments of Penicillium chrysogenum mycophage is unique.

AB - The base composition dependence of doublestranded ribonucleic acid (RNA) melting was studied by observing the structure and widths of melting transitions for Penicillium chrysogenum mycophage RNA as well as differences in melting temperatures of two RNAs of different base composition. Double-stranded RNA melting is independent of base compositions in 3.5 M Et4NCl and 4.6 M Me4NCl, where the melting temperatures are 25 and 92 °C, respectively. Double-stranded RNA renaturation rate constants are reported in Et4NCl solutions. Th e nucleation rate constant is about 10 times lower than that for double-stranded deoxyribonucleic acid. Analyses of renaturation kinetics results lead to the conclusion that each of the three similar but separable RNA segments of Penicillium chrysogenum mycophage is unique.

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JO - Biochemistry

JF - Biochemistry

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ER -

Denaturation and Renaturation of Penicillium chrysogenum Mycophage Double-Stranded Ribonucleic Acid in Tetraalkylammonium Salt Solutions

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