Delivery of modified mrna in a myocardial infarction mouse model

Keerat Kaur, Nishat Sultana, Yoav Hadas, Ajit Magadum, Mohammad Tofael Kabir Sharkar, Elena Chepurko, Lior Zangi

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Myocardial infarction (MI) is a leading cause of morbidity and mortality in the Western world. In the past decade, gene therapy has become a promising treatment option for heart disease, owing to its efficiency and exceptional therapeutic effects. In an effort to repair the damaged tissue post-MI, various studies have employed DNA-based or viral gene therapy but have faced considerable hurdles due to the poor and uncontrolled expression of the delivered genes, edema, arrhythmia, and cardiac hypertrophy. Synthetic modified mRNA (modRNA) presents a novel gene therapy approach that offers high, transient, safe, nonimmunogenic, and controlled mRNA delivery to the heart tissue without any risk of genomic integration. Due to these remarkable characteristics combined with its bell-shaped pharmacokinetics in the heart, modRNA has become an attractive approach for the treatment of heart disease. However, to increase its effectiveness in vivo, a consistent and reliable delivery method needs to be followed. Hence, to maximize modRNA delivery efficiency and yield consistency in modRNA use for in vivo applications, an optimized method of preparation and delivery of modRNA intracardiac injection in a mouse MI model is presented. This protocol will make modRNA delivery more accessible for basic and translational research.

Original languageEnglish
Article numbere60832
Pages (from-to)1-9
Number of pages9
JournalJournal of Visualized Experiments
Volume2020
Issue number160
DOIs
StatePublished - Jun 2020

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