Delivery of a vector encoding mouse hyaluronan synthase 2 via a crosslinked hyaluronan film

Angela P. Kim; Paige Yellen; Yang H. Yun; Evren Azeloglu; Weiliam Chen

doi:10.1016/j.biomaterials.2004.05.015

Delivery of a vector encoding mouse hyaluronan synthase 2 via a crosslinked hyaluronan film

Angela P. Kim, Paige Yellen, Yang H. Yun, Evren Azeloglu, Weiliam Chen

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We have developed a crosslinked hyaluronic acid (HA) film with DNA incorporated within its structure and have characterized this system for its efficacy in sustained transferring of a vector encoding mouse hyaluronan synthase 2 (Has2). Analysis of the DNA release kinetics indicated that the HA films degraded when treated with hyaluronidase and that they released DNA over a prolonged period of time. Gel electrophoresis revealed that this DNA was intact and immunohistochemical analysis verified the transfection capabilities of DNA release samples. The ability of released DNA encoding Has2 to promote HA synthesis was confirmed by quantifying the amount of HA produced by COS-1 cells that were transfected with release samples. The intended future application of the HA films is in prevention of post-operative peritoneal adhesions. In addition to serving as a physical barrier, the film would function as a vehicle for sustained delivery of DNA encoding Has2, which would promote the synthesis of HA in transfected tissues.

Original language	English
Pages (from-to)	1585-1593
Number of pages	9
Journal	Biomaterials
Volume	26
Issue number	13
DOIs	https://doi.org/10.1016/j.biomaterials.2004.05.015
State	Published - May 2005
Externally published	Yes

Keywords

Gene delivery
HA synthase
Hyaluronic acid
Post-operative adhesions

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10.1016/j.biomaterials.2004.05.015

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title = "Delivery of a vector encoding mouse hyaluronan synthase 2 via a crosslinked hyaluronan film",

abstract = "We have developed a crosslinked hyaluronic acid (HA) film with DNA incorporated within its structure and have characterized this system for its efficacy in sustained transferring of a vector encoding mouse hyaluronan synthase 2 (Has2). Analysis of the DNA release kinetics indicated that the HA films degraded when treated with hyaluronidase and that they released DNA over a prolonged period of time. Gel electrophoresis revealed that this DNA was intact and immunohistochemical analysis verified the transfection capabilities of DNA release samples. The ability of released DNA encoding Has2 to promote HA synthesis was confirmed by quantifying the amount of HA produced by COS-1 cells that were transfected with release samples. The intended future application of the HA films is in prevention of post-operative peritoneal adhesions. In addition to serving as a physical barrier, the film would function as a vehicle for sustained delivery of DNA encoding Has2, which would promote the synthesis of HA in transfected tissues.",

keywords = "Gene delivery, HA synthase, Hyaluronic acid, Post-operative adhesions",

author = "Kim, {Angela P.} and Paige Yellen and Yun, {Yang H.} and Evren Azeloglu and Weiliam Chen",

note = "Funding Information: This study was supported by a grant (HL66876, WC) from the National Institutes of Health. Partial support was also provided by HL65175 (WC). We would also like to thank Dr. Partap Khalsa and Ms. Jesse Little for their assistance in the measurement of the mechanical properties of the HA films, Dr. John A. McDonald (University of Utah, Salt Lake City) for providing the Has2 template for PCR, and Dan M. Checkla and Steven D{\textquoteright}Amico (Clear Solutions Biotech, Inc.) for their preparation of the Has2 plasmid construct used in this study.",

year = "2005",

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doi = "10.1016/j.biomaterials.2004.05.015",

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pages = "1585--1593",

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AU - Kim, Angela P.

AU - Yellen, Paige

AU - Yun, Yang H.

AU - Azeloglu, Evren

AU - Chen, Weiliam

N1 - Funding Information: This study was supported by a grant (HL66876, WC) from the National Institutes of Health. Partial support was also provided by HL65175 (WC). We would also like to thank Dr. Partap Khalsa and Ms. Jesse Little for their assistance in the measurement of the mechanical properties of the HA films, Dr. John A. McDonald (University of Utah, Salt Lake City) for providing the Has2 template for PCR, and Dan M. Checkla and Steven D’Amico (Clear Solutions Biotech, Inc.) for their preparation of the Has2 plasmid construct used in this study.

PY - 2005/5

Y1 - 2005/5

N2 - We have developed a crosslinked hyaluronic acid (HA) film with DNA incorporated within its structure and have characterized this system for its efficacy in sustained transferring of a vector encoding mouse hyaluronan synthase 2 (Has2). Analysis of the DNA release kinetics indicated that the HA films degraded when treated with hyaluronidase and that they released DNA over a prolonged period of time. Gel electrophoresis revealed that this DNA was intact and immunohistochemical analysis verified the transfection capabilities of DNA release samples. The ability of released DNA encoding Has2 to promote HA synthesis was confirmed by quantifying the amount of HA produced by COS-1 cells that were transfected with release samples. The intended future application of the HA films is in prevention of post-operative peritoneal adhesions. In addition to serving as a physical barrier, the film would function as a vehicle for sustained delivery of DNA encoding Has2, which would promote the synthesis of HA in transfected tissues.

AB - We have developed a crosslinked hyaluronic acid (HA) film with DNA incorporated within its structure and have characterized this system for its efficacy in sustained transferring of a vector encoding mouse hyaluronan synthase 2 (Has2). Analysis of the DNA release kinetics indicated that the HA films degraded when treated with hyaluronidase and that they released DNA over a prolonged period of time. Gel electrophoresis revealed that this DNA was intact and immunohistochemical analysis verified the transfection capabilities of DNA release samples. The ability of released DNA encoding Has2 to promote HA synthesis was confirmed by quantifying the amount of HA produced by COS-1 cells that were transfected with release samples. The intended future application of the HA films is in prevention of post-operative peritoneal adhesions. In addition to serving as a physical barrier, the film would function as a vehicle for sustained delivery of DNA encoding Has2, which would promote the synthesis of HA in transfected tissues.

KW - Gene delivery

KW - HA synthase

KW - Hyaluronic acid

KW - Post-operative adhesions

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JO - Biomaterials

JF - Biomaterials

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ER -

Delivery of a vector encoding mouse hyaluronan synthase 2 via a crosslinked hyaluronan film

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Keywords

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