TY - JOUR
T1 - Defects in hemostasis in P-selectin-deficient mice
AU - Subramaniam, Meera
AU - Frenette, Paul S.
AU - Saffaripour, Simin
AU - Johnson, Robert C.
AU - Hynes, Richard O.
AU - Wagner, Denisa D.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Recently, our laboratory showed that platelets, like leukocytes, roll on activated endothelium expressing P-selectin, thus suggesting a role for P- selectin in hemostasis (Frenette et al, Proc Natl Acad Sci USA 92:7450, 1995). We report here that the P-selectin-deficient mice show a 40% prolongation of the bleeding time on amputation of the tip of the tail. Moreover, defective hemostasis was observed in a local Shwartzman-like reaction induced by skin injections of lipopolysaccharide followed by tumor necrosis factor-α in the P-selectin-deficient mice. The hemorrhagic lesions, quantitated both macroscopically end microscopically, were twofold larger in the P-selectin-deficient mice. This was also confirmed by measuring the radioactivity in the skin using chromium-labeled red blood cells. Therefore, it is evident that P-selectin plays a role in hemostasis as suggested by its support of platelet rolling.
AB - Recently, our laboratory showed that platelets, like leukocytes, roll on activated endothelium expressing P-selectin, thus suggesting a role for P- selectin in hemostasis (Frenette et al, Proc Natl Acad Sci USA 92:7450, 1995). We report here that the P-selectin-deficient mice show a 40% prolongation of the bleeding time on amputation of the tip of the tail. Moreover, defective hemostasis was observed in a local Shwartzman-like reaction induced by skin injections of lipopolysaccharide followed by tumor necrosis factor-α in the P-selectin-deficient mice. The hemorrhagic lesions, quantitated both macroscopically end microscopically, were twofold larger in the P-selectin-deficient mice. This was also confirmed by measuring the radioactivity in the skin using chromium-labeled red blood cells. Therefore, it is evident that P-selectin plays a role in hemostasis as suggested by its support of platelet rolling.
UR - http://www.scopus.com/inward/record.url?scp=0030049002&partnerID=8YFLogxK
U2 - 10.1182/blood.v87.4.1238.bloodjournal8741238
DO - 10.1182/blood.v87.4.1238.bloodjournal8741238
M3 - Article
C2 - 8608210
AN - SCOPUS:0030049002
SN - 0006-4971
VL - 87
SP - 1238
EP - 1242
JO - Blood
JF - Blood
IS - 4
ER -