Defects in hemostasis in P-selectin-deficient mice

Meera Subramaniam, Paul S. Frenette, Simin Saffaripour, Robert C. Johnson, Richard O. Hynes, Denisa D. Wagner

Research output: Contribution to journalArticlepeer-review

179 Scopus citations


Recently, our laboratory showed that platelets, like leukocytes, roll on activated endothelium expressing P-selectin, thus suggesting a role for P- selectin in hemostasis (Frenette et al, Proc Natl Acad Sci USA 92:7450, 1995). We report here that the P-selectin-deficient mice show a 40% prolongation of the bleeding time on amputation of the tip of the tail. Moreover, defective hemostasis was observed in a local Shwartzman-like reaction induced by skin injections of lipopolysaccharide followed by tumor necrosis factor-α in the P-selectin-deficient mice. The hemorrhagic lesions, quantitated both macroscopically end microscopically, were twofold larger in the P-selectin-deficient mice. This was also confirmed by measuring the radioactivity in the skin using chromium-labeled red blood cells. Therefore, it is evident that P-selectin plays a role in hemostasis as suggested by its support of platelet rolling.

Original languageEnglish
Pages (from-to)1238-1242
Number of pages5
Issue number4
StatePublished - 15 Feb 1996


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