Defective glycosylphosphatidylinositol anchor synthesis in paroxysmal nocturnal hemoglobinuria granulocytes

John F. Mahoney, Masaharu Urakaze, Sharon Hall, Rita DeGasperi, Hui Ming Chang, Eiji Sugiyama, Christopher D. Warren, Michael Borowitz, Anne Nicholson-Weller, Wendell F. Rosse, Edward T.H. Yeh

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


To investigate the biosynthesis of the glycosylphosphatidylinositol (GPI) anchor in the granulocytes of paroxysmal nocturnal hemoglobinuria (PNH), the glycolipids of granulocytes from PNH patients and normal volunteers were biosynthetically labeled with [3H]mannose in the presence of tunicamycin. Extracted glycolipids were examined by thin-layer chromatography and compared with known biosynthetic intermediates. Normal granulocytes consistently showed [3H]mannose incorporation into the complete GPI core, several GPI biosynthetic intermediates, and dolichol phosphate mannose (DPM). The granulocytes of 10 patients with PNH that had no expression of CD55 and CD59 on greater than 95% of the cells were able to incorporate [3H]mannose into DPM, but were not able to incorporate detectable amounts into the complete GPI core. Thus, PNH granulocytes do not synthesize detectable amounts of the complete GPI core and this defect likely accounts for the absence of GPI-linked membrane proteins on hematopoietic cells in this syndrome.

Original languageEnglish
Pages (from-to)1400-1403
Number of pages4
Issue number6
StatePublished - 15 Mar 1992


Dive into the research topics of 'Defective glycosylphosphatidylinositol anchor synthesis in paroxysmal nocturnal hemoglobinuria granulocytes'. Together they form a unique fingerprint.

Cite this