Dectin-1 expression at early period of Aspergillus fumigatus infection in rat's corneal epithelium

Cheng Ye Che, Cui Li, Ang Gao, Jing Lin, Li Li Zhang, Qiang Xu, Qian Wang, Gui Qiu Zhao

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

AIM: To investigate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) at the early period of Aspergillus fumigatus infection in rat's corneal epithelium. METHODS: A total of 72 Wistar rats were randomly divided into three groups: A, B and C. The right eyes were chosen as experimental eyes. Group A was control group. Rats in group B were not inoculated with Aspergillus fumigatus. Group C was taken as Aspergillus fumigatus keratitis model. Rats in group B and C (six from each group) were executed randomly at 4, 8, 16 and 24 hours after experimental model being established to assess the expression of dectin-1 m RNA through real-time PCR. Another six rats in group B and C were executed randomly at 24 hours to assess the expression of dectin-1 protein through immunohistochemistry. RESULTS: The results of real-time PCR indicated that dectin-1 m RNA expression was low in corneal epithelium of normal rats'. There was no significantly difference of dectin-1 m RNA expression in group A and B (P>0.05). The expression of Aspergillus fumigatus infected corneal epithelium increased gradually after 8 hours in group C. The synchronous expression of group A and C had significant difference (P < 0.01). Immunohistochemisty discovered that dectin-1 receptor existed in normal rat's corneal epithelium. Dectin-1 protein increased after 24 hours in group C. There was a significant difference of synchronous expression in group B and C (P < 0.01). CONCLUSION: Dectin-1 exists in rat's corneal epithelium and its expression significantly increases at the early period of Aspergillus fumigatus infection. Dectin-1 is a pattern recognition receptor that expresses in corneal epithelium and in volves in immune response to Aspergillus fungal keratitis. Copyright International Journal of Ophthalmology Press.

Original languageEnglish
Pages (from-to)30-33
Number of pages4
JournalInternational Journal of Ophthalmology
Volume6
Issue number1
DOIs
StatePublished - 2013
Externally publishedYes

Keywords

  • Aspergillus fumigatus
  • Dectin-1
  • Keratitis
  • Rat

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