Decreased dopamine uptake into platelet storage granules in Gilles de la Tourette disease

Jose Martin Rabey; Ziona Oberman; Eran Graff; Amos D. Korczyn

doi:10.1016/0006-3223(94)00234-T

Decreased dopamine uptake into platelet storage granules in Gilles de la Tourette disease

Jose Martin Rabey, Ziona Oberman, Eran Graff, Amos D. Korczyn

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The movement disorder of Gilles de la Tourette (GdlT) disease may reflect hyperactivity of the basal ganglionic dopamine system. Since platelets have been suggested as peripheral models for the study of catecholamine neurons, we developed a method to measure the uptake of [³H]-DA into platelet storage granules (PSG). In the present report, PSG were incubated with [³H] DA, and V_max and Km values were calculated by linear regression analysis (Lineweaver Burke plot). The uptake of DA (0.5-5 μM) by PSG from 18 GdlT patients was significantly lower (p < .0001) compared to 15 controls (V_max mean ± SD, 107.5 ± 42.5 and 265.3 ± 66.5 fmole/mg protein resp.). The decrease of DA uptake in GdlT may reflect compensatory presynaptic changes that reduce DA activity.

Original language	English
Pages (from-to)	112-115
Number of pages	4
Journal	Biological Psychiatry
Volume	38
Issue number	2
DOIs	https://doi.org/10.1016/0006-3223(94)00234-T
State	Published - 15 Jul 1995
Externally published	Yes

Keywords

Platelets
Tourette
dopamine
platelet storage granules

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10.1016/0006-3223(94)00234-T

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title = "Decreased dopamine uptake into platelet storage granules in Gilles de la Tourette disease",

abstract = "The movement disorder of Gilles de la Tourette (GdlT) disease may reflect hyperactivity of the basal ganglionic dopamine system. Since platelets have been suggested as peripheral models for the study of catecholamine neurons, we developed a method to measure the uptake of [3H]-DA into platelet storage granules (PSG). In the present report, PSG were incubated with [3H] DA, and Vmax and Km values were calculated by linear regression analysis (Lineweaver Burke plot). The uptake of DA (0.5-5 μM) by PSG from 18 GdlT patients was significantly lower (p < .0001) compared to 15 controls (Vmax mean ± SD, 107.5 ± 42.5 and 265.3 ± 66.5 fmole/mg protein resp.). The decrease of DA uptake in GdlT may reflect compensatory presynaptic changes that reduce DA activity.",

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doi = "10.1016/0006-3223(94)00234-T",

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T1 - Decreased dopamine uptake into platelet storage granules in Gilles de la Tourette disease

AU - Rabey, Jose Martin

AU - Oberman, Ziona

AU - Graff, Eran

AU - Korczyn, Amos D.

PY - 1995/7/15

Y1 - 1995/7/15

N2 - The movement disorder of Gilles de la Tourette (GdlT) disease may reflect hyperactivity of the basal ganglionic dopamine system. Since platelets have been suggested as peripheral models for the study of catecholamine neurons, we developed a method to measure the uptake of [3H]-DA into platelet storage granules (PSG). In the present report, PSG were incubated with [3H] DA, and Vmax and Km values were calculated by linear regression analysis (Lineweaver Burke plot). The uptake of DA (0.5-5 μM) by PSG from 18 GdlT patients was significantly lower (p < .0001) compared to 15 controls (Vmax mean ± SD, 107.5 ± 42.5 and 265.3 ± 66.5 fmole/mg protein resp.). The decrease of DA uptake in GdlT may reflect compensatory presynaptic changes that reduce DA activity.

AB - The movement disorder of Gilles de la Tourette (GdlT) disease may reflect hyperactivity of the basal ganglionic dopamine system. Since platelets have been suggested as peripheral models for the study of catecholamine neurons, we developed a method to measure the uptake of [3H]-DA into platelet storage granules (PSG). In the present report, PSG were incubated with [3H] DA, and Vmax and Km values were calculated by linear regression analysis (Lineweaver Burke plot). The uptake of DA (0.5-5 μM) by PSG from 18 GdlT patients was significantly lower (p < .0001) compared to 15 controls (Vmax mean ± SD, 107.5 ± 42.5 and 265.3 ± 66.5 fmole/mg protein resp.). The decrease of DA uptake in GdlT may reflect compensatory presynaptic changes that reduce DA activity.

KW - Platelets

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KW - dopamine

KW - platelet storage granules

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Decreased dopamine uptake into platelet storage granules in Gilles de la Tourette disease

Abstract

Keywords

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